Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Disease Control and Prevention, Dali 671000, China.
State Key Laboratory for Biocontrol, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
Viruses. 2024 Apr 25;16(5):674. doi: 10.3390/v16050674.
Yunnan province in China shares its borders with three neighboring countries: Myanmar, Vietnam, and Laos. The region is characterized by a diverse climate and is known to be a suitable habitat for various arthropods, including midges which are notorious for transmitting diseases which pose significant health burdens affecting both human and animal health. A total of 431,100 midges were collected from 15 different locations in the border region of Yunnan province from 2015 to 2020. These midges were divided into 37 groups according to the collection year and sampling site. These 37 groups of midges were then homogenized to extract nucleic acid. Metatranscriptomics were used to analyze their viromes. Based on the obtained cytochrome C oxidase I gene (COI) sequences, three genera were identified, including one species of , one species of and twenty-five species of . We identified a total of 3199 viruses in five orders and 12 families, including 1305 single-stranded positive-stranded RNA viruses (+ssRNA) in two orders and seven families, 175 single-stranded negative-stranded RNA viruses (-ssRNA) in two orders and one family, and 1719 double-stranded RNA viruses in five families. Six arboviruses of economic importance were identified, namely Banna virus (BAV), Japanese encephalitis virus (JEV), Akabane virus (AKV), Bluetongue virus (BTV), Tibetan circovirus (TIBOV), and Epizootic hemorrhagic disease virus (EHDV), all of which are capable, to varying extents, of causing disease in humans and/or animals. The survey sites in this study basically covered the current distribution area of midges in Yunnan province, which helps to predict the geographic expansion of midge species. The complexity and diversity of the viral spectrum carried by midges identified in the study calls for more in-depth research, which can be utilized to monitor arthropod vectors and to predict the emergence and spread of zoonoses and animal epidemics, which is of great significance for the control of vector-borne diseases.
缅甸、越南和老挝。该地区气候多样，是各种节肢动物（包括传播疾病的蚊虫）的适宜栖息地，这些疾病对人类和动物的健康都构成了重大健康负担。2015 年至 2020 年，从云南省边境地区的 15 个不同地点共采集了 431100 只蚊虫。根据采集年份和采样地点，将这些蚊虫分为 37 组。然后将这 37 组蚊虫均质化以提取核酸。对其病毒组进行了宏转录组学分析。根据获得的细胞色素 C 氧化酶 I 基因（COI）序列，鉴定出三个属，包括一种 、一种 和 25 种 。我们在五个目和 12 个科中共鉴定出 3199 种病毒，包括两个目中的 1305 种单链正链 RNA 病毒（+ssRNA）和七个科中的 75 种单链负链 RNA 病毒（-ssRNA），以及五个科中的 1719 种双链 RNA 病毒。鉴定出六种具有经济重要性的虫媒病毒，即 Banna 病毒（BAV）、日本脑炎病毒（JEV）、Akabane 病毒（AKV）、蓝舌病病毒（BTV）、西藏圆环病毒（TIBOV）和出血性疾病病毒（EHDV），这些病毒都能在不同程度上导致人类和/或动物患病。本研究的调查地点基本涵盖了云南省目前蚊虫的分布区域，有助于预测蚊虫物种的地理扩张。本研究中鉴定的蚊虫携带的病毒谱的复杂性和多样性需要更深入的研究，可以用于监测节肢动物媒介，并预测人畜共患病和动物流行病的出现和传播，这对于控制虫媒疾病具有重要意义。
