Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Dr., Madison, WI 53711, USA.
J Virol. 2010 Apr;84(7):3699-706. doi: 10.1128/JVI.02255-09. Epub 2010 Jan 20.
Here we describe a novel vaccine vector for expressing human immunodeficiency virus (HIV) antigens. We show that recombinant attenuated yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 Gag sequences can be used as a vector to generate SIV-specific CD8(+) T-cell responses in the rhesus macaque. Priming with recombinant BCG expressing SIV antigens increased the frequency of these SIV-specific CD8(+) T-cell responses after recombinant YF17D boosting. These recombinant YF17D-induced SIV-specific CD8(+) T cells secreted several cytokines, were largely effector memory T cells, and suppressed viral replication in CD4(+) T cells.
在这里，我们描述了一种用于表达人类免疫缺陷病毒 (HIV) 抗原的新型疫苗载体。我们表明，表达猿猴免疫缺陷病毒 SIVmac239 Gag 序列的重组减毒黄热疫苗病毒 17D 可用作载体，在恒河猴中产生针对 SIV 的特异性 CD8(+) T 细胞反应。用表达 SIV 抗原的重组卡介苗进行初免后，用重组 YF17D 加强免疫可增加这些针对 SIV 的特异性 CD8(+) T 细胞反应的频率。这些重组 YF17D 诱导的 SIV 特异性 CD8(+) T 细胞分泌几种细胞因子，主要是效应记忆 T 细胞，并抑制 CD4(+) T 细胞中的病毒复制。
