产超广谱β-内酰胺酶革兰氏阴性菌的质粒图谱及其与β-内酰胺类药物敏感性的相关性。
Plasmid profile of ESBL producing Gram-negative bacteria and correlation with susceptibility to beta-lactam drugs.
作者信息
Sharma Jyoti, Ray Pallab, Sharma Meera
机构信息
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
Indian J Pathol Microbiol. 2010 Jan-Mar;53(1):83-6. doi: 10.4103/0377-4929.59190.
BACKGROUND
Members of family Enterobacteriaceae can acquire resistance to extended spectrum beta lactams by a number of mechanisms; most important being the plasmid encoded extended spectrum beta lactamase (ESBL) and AmpC beta lactamase. This study has been designed to look for the presence of plasmids and their correlation with drug resistance.
METHODS
ESBL production was studied in different gram-negative bacteria and susceptibility testing of ESBL positive isolates was done for various beta lactams, cephalosporins and other commonly used drugs against them. Plasmid DNA isolation of all the ESBL positive strains was done by alkalilysis method. Finally the presence of plasmid was correlated with susceptibility to beta lactam drugs.
RESULTS
E. coli, K. pneumoniae, Enterobacter aerogenes and A. anitratus harbored multiple plasmids. One plasmid (M.W greater than 21,226 bp) was unanimously present in all the isolates.
CONCLUSION
There is a strong correlation between the number of plasmids harbored by an isolate and resistance to various drugs tested.
背景
肠杆菌科成员可通过多种机制获得对超广谱β-内酰胺类药物的耐药性；其中最重要的是质粒编码的超广谱β-内酰胺酶（ESBL）和AmpCβ-内酰胺酶。本研究旨在寻找质粒的存在及其与耐药性的相关性。
方法
对不同革兰氏阴性菌进行ESBL产生情况的研究，并对ESBL阳性分离株针对各种β-内酰胺类、头孢菌素类及其他常用药物进行药敏试验。所有ESBL阳性菌株的质粒DNA提取采用碱裂解法。最后将质粒的存在与对β-内酰胺类药物的敏感性进行相关性分析。
结果
大肠杆菌、肺炎克雷伯菌、产气肠杆菌和阴沟肠杆菌携带多个质粒。所有分离株中均一致存在一种质粒（分子量大于21,226 bp）。
结论
分离株携带的质粒数量与所测试的各种药物的耐药性之间存在很强的相关性。
Zotero 插件