Akinduti Paul Akinniyi, Olasehinde Grace Iyabo, Ejilude Oluwaseun, Taiwo Olugbenga Samson, Obafemi Yemisi Dorcas
Microbiology Unit, Department of Biological Sciences, Covenant University, Otta, Nigeria,
Microbiology Unit, Sacred Heart Hospital, Lantoro, Abeokuta, Nigeria.
Infect Drug Resist. 2018 Nov 27;11:2425-2433. doi: 10.2147/IDR.S178243. eCollection 2018.
Increasing rates of clonal spread of fecal bacilli remains a huge concern to the community health with resultant high morbidity. The fecal carriage and clonal diversity of within the communities in Southwest Nigeria were surveyed.
Enteric bacilli obtained from fresh fecal samples randomly collected from community residents were biotyped and profiled for antibiotic susceptibility. Resistant strains were typed for beta-lactamase, extended-spectrum beta-lactamases (ESBL), AmpC and carbapenemase production while the R-plasmid carriage was detected and mating activities were examined. The presence of gene was assayed by PCR and its phylodiversity determined with 16sRNA genomic profiling.
have the highest (28.6%) occurrence rate and (20.5%) showing significant resistance to beta-lactamase inhibitors (ampicillin, cefuroxime and cefotaxime), and high-level multidrug resistance of more than 15.2% rate to ampicillin, cefuroxime, ceftazidime, tetracycline and imipenem. and , are the highest beta-lactamase, ESBL and AmpC producers encoded with high molecular weight R-plasmid (>11.0 kbp) and significant rate of conjugation and transformational activities. Only 2/14, 1/13 and 1/6 ESBL-type of , and expressed gene, clustering into five different phylodiverse groups with close genomic relatedness with other bacilli.
This is an indication of clonal dissemination of ESBL encoded enteric bacilli having high phylodiverse characteristics through fecal carriage in the Nigerian community which requires public health education, food and environmental hygiene for its prevention.
粪便杆菌克隆传播率的不断上升仍是社区健康的一大担忧，会导致高发病率。对尼日利亚西南部社区内粪便杆菌的携带情况和克隆多样性进行了调查。
从社区居民随机采集的新鲜粪便样本中获取肠道杆菌，进行生物分型并分析抗生素敏感性。对耐药菌株进行β-内酰胺酶、超广谱β-内酰胺酶（ESBL）、AmpC和碳青霉烯酶产生情况的分型，同时检测R质粒携带情况并检查接合活性。通过PCR检测基因的存在，并通过16sRNA基因组分析确定其系统发育多样性。
具有最高（28.6%）的发生率，且（20.5%）对β-内酰胺酶抑制剂（氨苄西林、头孢呋辛和头孢噻肟）表现出显著耐药性，对氨苄西林、头孢呋辛、头孢他啶、四环素和亚胺培南的高水平多重耐药率超过15.2%。和是最高产β-内酰胺酶、ESBL和AmpC的菌株，编码有高分子量R质粒（>11.0 kbp），且接合和转化活性率较高。只有2/14、1/13和1/6的ESBL型、和表达基因，聚类为五个不同的系统发育多样组，与其他杆菌具有密切的基因组相关性。
这表明在尼日利亚社区中，通过粪便携带具有高系统发育多样性特征的ESBL编码肠道杆菌存在克隆传播，需要进行公共卫生教育、食品和环境卫生以预防。
