Maki Nobuyasu, Martinson John, Nishimura Osamu, Tarui Hiroshi, Meller Jaroslaw, Tsonis Panagiotis A, Agata Kiyokazu
Center for Developmental Biology, RIKEN Kobe, Kobe, Japan.
Mol Vis. 2010 Jan 18;16:72-8.
The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level.
mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed.
From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found.
The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).
成年蝾螈可通过去分化从背侧虹膜的色素上皮细胞（PEC）再生晶状体。本研究的目的是获取蝾螈晶状体再生研究的序列资源，并在分子水平上深入了解去分化过程。
在去分化过程中从虹膜中纯化mRNA，并构建其cDNA文库。对cDNA文库中的10449个克隆进行测序和分析。
从10449条读数中注释出780个重叠群和1666个单拷贝序列。揭示了蝾螈去分化过程中几种与癌症和凋亡相关基因的存在。此外，还发现了几个可能参与去分化过程中重编程的候选基因。
癌症和凋亡相关基因的表达可能是去分化过程中的标志。表达序列标签（EST）资源对蝾螈去分化的未来研究有用，序列信息可在GenBank中获取（登录号：FS290155 - FS300559）。
