Malloch Erica L, Perry Kimberly J, Fukui Lisa, Johnson Verity R, Wever Jason, Beck Caroline W, King Michael W, Henry Jonathan J
University of Illinois, Department of Cell and Developmental Biology, Urbana, Illinois 61801, USA.
Dev Dyn. 2009 Sep;238(9):2340-56. doi: 10.1002/dvdy.21998.
Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected.
从一个富集了非洲爪蟾晶状体再生过程中上调基因的cDNA文库中，获得了734个独特的基因。这些序列代表转录因子、参与RNA合成/加工的蛋白质、主要细胞信号通路的组分、参与蛋白质加工、运输和降解的基因（例如泛素/蛋白酶体途径）、基质金属蛋白酶（MMPs）以及许多其他蛋白质。这些发现表明特定的信号转导途径参与晶状体再生过程，包括FGF、TGF-β、MAPK、视黄酸、Wnt和刺猬信号通路，已知这些通路在各种系统的眼/晶状体发育和再生中发挥重要作用。原位杂交显示，回收的大多数基因在胚胎发育过程中表达，包括在眼组织中。鉴定出了几个在晶状体中特异性表达的新基因。将该组基因与其他再生组织/生物体中上调的基因进行比较，发现有少量重叠。
