Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, Japan.
J Nat Med. 2010 Jan;64(1):117-20. doi: 10.1007/s11418-009-0372-2. Epub 2009 Nov 7.
The anti-influenza A/PR/8/34 (H1N1) virus activities of ten diarylheptanoids isolated from Alpinia officinarum were examined using the MTT method. The 50% inhibitory concentration of each diarylheptanoid examined was clearly lower than its 50% cytotoxic concentration determined by the MTT assay and/or maximum non-cytotoxic concentration (MNCC) determined by the morphological change of cells. In particular, the influenza virus was more susceptible to 7-(4''-hydroxy-3''-methoxyphenyl)-1-phenyl-4E-hepten-3-one (3) and (5S)-5-hydroxy-7-(4''-hydroxyphenyl)-1-phenyl-3-heptanone (8) than the other diarylheptanoids. Thus, all diarylheptanoids exhibited potential antiviral activity against influenza virus in vitro.
采用 MTT 法检测了从高良姜中分离得到的 10 种二芳基庚烷类化合物对甲型/PR/8/34（H1N1）流感病毒的抑制活性。各二芳基庚烷类化合物的 50%抑制浓度均明显低于其通过 MTT 测定的 50%细胞毒性浓度和/或通过细胞形态变化确定的最大非细胞毒性浓度（MNCC）。特别是，与其他二芳基庚烷类化合物相比，7-(4''-羟基-3''-甲氧基苯基)-1-苯基-4E-庚烯-3-酮（3）和(5S)-5-羟基-7-(4''-羟基苯基)-1-苯基-3-庚酮（8）对流感病毒的敏感性更高。因此，所有二芳基庚烷类化合物在体外均表现出抗流感病毒的潜在活性。
