Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba 283-8555, Japan.
School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Evid Based Complement Alternat Med. 2014;2014:204797. doi: 10.1155/2014/204797. Epub 2014 Aug 31.
Diarylheptanoids (AO-0001, AO-0002, and AO-0003) isolated from Alpinia officinarum inhibit proinflammatory mediators and exhibit cytotoxic and antiviral activity. However, the precise mechanisms of action of these diarylheptanoids are unknown as are their effects on expression of specific genes. Here, we used a translatome analysis to investigate the mechanisms and modes of action of these three diarylheptanoids. Polysome-associated messenger RNAs (mRNAs) were prepared from diarylheptanoids-treated and control cells from a human B lymphoblastoid cell line; these mRNA samples were then used for microarray analysis. Microarray Data Analysis Tool version 3.2 was used to analyze the microarray data analysis; this software uses pathway information of the WikiPathways for gene ontology analysis. Each of the diarylheptanoids caused upregulation or downregulation of the same 37 and 286 genes, respectively. Among the 37 upregulated genes, 16 were related to mRNA processing based on the WikiPathways analysis. Our findings provided new insights into the mode of action of diarylheptanoids from A. officinarum.
从高良姜中分离得到的二芳基庚烷类化合物（AO-0001、AO-0002 和 AO-0003）可抑制促炎介质的产生，并具有细胞毒性和抗病毒活性。然而，这些二芳基庚烷类化合物的确切作用机制及其对特定基因表达的影响尚不清楚。在这里，我们使用翻译组分析来研究这三种二芳基庚烷类化合物的作用机制和作用模式。从人 B 淋巴母细胞系中用二芳基庚烷类化合物处理和对照细胞制备多核糖体相关信使 RNA (mRNA)；然后使用这些 mRNA 样品进行微阵列分析。微阵列数据分析工具版本 3.2 用于微阵列数据分析；该软件使用 WikiPathways 的途径信息进行基因本体分析。每种二芳基庚烷类化合物分别导致相同的 37 个和 286 个基因的上调或下调。在 37 个上调的基因中，根据 WikiPathways 分析，有 16 个与 mRNA 加工有关。我们的研究结果为高良姜中二芳基庚烷类化合物的作用模式提供了新的见解。
