School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK.
J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):128-40. doi: 10.1002/jbm.b.31567.
Contemporary medical science is reliant upon the rational selection and utilization of devices, and therefore, an increasing need has developed for in vitro systems aimed at replicating the conditions to which urological devices will be subjected to during their use in vivo. We report the development and validation of a novel continuous flow encrustation model based on the commercially available CDC biofilm reactor. Proteus mirabilis-induced encrustation formation on test biomaterial sections under varying experimental parameters was analyzed by X-ray diffraction, infrared- and Raman spectroscopy and by scanning electron microscopy. The model system produced encrusted deposits similar to those observed in archived clinical samples. Results obtained for the system are highly reproducible with encrustation being rapidly deposited on test biomaterial sections. This model will have utility in the rapid screening of encrustation behavior of biomaterials for use in urological applications.
当代医学科学依赖于设备的合理选择和利用，因此，越来越需要开发旨在复制泌尿科设备在体内使用时所经历条件的体外系统。我们报告了一种新型连续流结垢模型的开发和验证，该模型基于市售的 CDC 生物膜反应器。通过 X 射线衍射、红外和拉曼光谱以及扫描电子显微镜分析了在不同实验参数下测试生物材料切片上由奇异变形杆菌诱导的结垢形成。模型系统产生的结垢沉积物类似于存档的临床样本中观察到的沉积物。该系统获得的结果具有高度重现性，可快速在测试生物材料切片上沉积结垢。该模型将可用于快速筛选用于泌尿科应用的生物材料的结垢行为。
