施氏假单胞菌 HPD-2 菌剂对老化污染土壤微生物群落及多环芳烃去除的影响。
Effect of bioaugmentation by Paracoccus sp. strain HPD-2 on the soil microbial community and removal of polycyclic aromatic hydrocarbons from an aged contaminated soil.
机构信息
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
出版信息
Bioresour Technol. 2010 May;101(10):3437-43. doi: 10.1016/j.biortech.2009.12.088. Epub 2010 Jan 21.
A microcosm study was conducted to test the bioremediation potential of Paracoccus sp. strain HPD-2 on an aged PAH-contaminated soil. Bioaugmented microcosms showed a 23.2% decrease in soil total PAH concentrations after 28days, with a decline in average concentration from 9942 to 7638microg kg(-1) dry soil. The percentage degradation of 3-, 4- and 5(+6)-ring PAHs was 35.1%, 20.7% and 24.3%, respectively. Higher counts of culturable PAH-degrading bacteria, microbial biomass and enzyme activities were observed in bioaugmented soil. The bioaugmented microcosms showed significant increases (p<0.05) in the average well-color development (AWCD) obtained by the BIOLOG ecoplate assay and Shannon-Weaver index (H) compared to the controls. Principal component analysis of BIOLOG data clearly differentiated between the bioaugmented and control microcosms, implying that bioaugmentation restored the microbiological functioning of the PAH-contaminated soil. The results suggest that bioaugmentation by Paracoccus sp. strain HPD-2 may be a promising bioremediation strategy for aged PAH-contaminated soils.
一项微观研究旨在测试 Paracoccus sp. 菌株 HPD-2 对老化多环芳烃污染土壤的生物修复潜力。经过 28 天，生物强化微宇宙显示土壤中总多环芳烃浓度下降了 23.2%，平均浓度从 9942 降至 7638μg kg(-1)干土。3、4 和 5(+6)环多环芳烃的降解率分别为 35.1%、20.7%和 24.3%。在生物强化土壤中观察到可培养多环芳烃降解菌、微生物生物量和酶活性的计数更高。与对照相比，生物强化微宇宙的 BIOLOG 生态板测定法获得的平均好氧显色发展（AWCD）和香农-威弗指数（H）均显著增加（p<0.05）。BIOLOG 数据的主成分分析清楚地区分了生物强化和对照微宇宙，这意味着生物强化恢复了多环芳烃污染土壤的微生物功能。结果表明，Paracoccus sp. 菌株 HPD-2 的生物强化可能是一种有前途的老化多环芳烃污染土壤的生物修复策略。
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