Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan.
Bioresour Technol. 2010 May;101(10):3595-601. doi: 10.1016/j.biortech.2009.12.084. Epub 2010 Jan 22.
The higher GlcN production using a wild-type fungi, Aspergillus sp. BCRC 31742 cultivated under submerged fermentation was investigated. Several fermentation aspects were studied, such as pellet size, working volume, agitation rate and stimulating factor. Culture cultivation with conditions, such as pellet diameter of 2.15mm, 50mL working volume (250mL T-flask), incubation at 30 degrees C, 200rpm and pH 7.0 for 5days yielded highest biomass concentration which was 33.82g/L, with a GlcN concentration of 7.05g/L. Methanol was found to give the best stimulatory effect in terms of GlcN concentration as compared to glutamic acid, cycloheximide and ethanol. Addition of methanol (1.5%v/v) into fermentation medium could increase GlcN content from 0.21 (control) to 0.26g/gdw cells and led to maximum GlcN concentration of 7.48g/L obtained.
研究了在液体深层发酵中利用野生型真菌 Aspergillus sp. BCRC 31742 提高 GlcN 产量的方法。考察了几种发酵方面的因素，如颗粒大小、工作体积、搅拌速度和刺激因子。在条件为：颗粒直径为 2.15mm、工作体积为 50mL（250mL T 烧瓶）、30°C 培养、200rpm 搅拌和 pH7.0 的条件下培养 5 天，可获得最高的生物量浓度 33.82g/L，GlcN 浓度为 7.05g/L。与谷氨酸、环己酰亚胺和乙醇相比，甲醇在 GlcN 浓度方面表现出最好的刺激效果。在发酵培养基中添加 1.5%（v/v）甲醇可将 GlcN 含量从 0.21（对照）提高到 0.26g/gdw 细胞，从而获得最高的 7.48g/L GlcN 浓度。
