合成生物学策略在改善微生物合成“绿色”生物聚合物方面的应用。
Synthetic biology strategies for improving microbial synthesis of "green" biopolymers.
机构信息
From the Department of Chemical Engineering and Center for Integrative Synthetic Biology (CISB), Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
From the Department of Chemical Engineering and Center for Integrative Synthetic Biology (CISB), Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
出版信息
J Biol Chem. 2018 Apr 6;293(14):5053-5061. doi: 10.1074/jbc.TM117.000368. Epub 2018 Jan 16.
Abstract
Polysaccharide-based biopolymers have many material properties relevant to industrial and medical uses, including as drug delivery agents, wound-healing adhesives, and food additives and stabilizers. Traditionally, polysaccharides are obtained from natural sources. Microbial synthesis offers an attractive alternative for sustainable production of tailored biopolymers. Here, we review synthetic biology strategies for select "green" biopolymers: cellulose, alginate, chitin, chitosan, and hyaluronan. Microbial production pathways, opportunities for pathway yield improvements, and advances in microbial engineering of biopolymers in various hosts are discussed. Taken together, microbial engineering has expanded the repertoire of green biological chemistry by increasing the diversity of biobased materials.
摘要
多糖基生物聚合物具有许多与工业和医疗用途相关的材料特性，包括作为药物传递剂、伤口愈合粘合剂以及食品添加剂和稳定剂。传统上，多糖是从天然来源中获得的。微生物合成提供了一种有吸引力的替代方法，可用于可持续生产定制的生物聚合物。在这里，我们回顾了选择的“绿色”生物聚合物的合成生物学策略：纤维素、藻酸盐、甲壳素、壳聚糖和透明质酸。讨论了微生物生产途径、途径产率提高的机会以及在各种宿主中生物聚合物的微生物工程学进展。总的来说，微生物工程通过增加生物基材料的多样性，扩展了绿色生物化学的范围。
相似文献
1
Synthetic biology strategies for improving microbial synthesis of "green" biopolymers.合成生物学策略在改善微生物合成“绿色”生物聚合物方面的应用。
J Biol Chem. 2018 Apr 6;293(14):5053-5061. doi: 10.1074/jbc.TM117.000368. Epub 2018 Jan 16.
2
Synthetic biology for microbial production of lipid-based biofuels.用于微生物生产脂质基生物燃料的合成生物学。
Curr Opin Chem Biol. 2015 Dec;29:58-65. doi: 10.1016/j.cbpa.2015.09.009. Epub 2015 Oct 23.
3
Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production.构建用于生产植物天然产物的微生物细胞工厂：从设计原理到工业规模生产
Microb Cell Fact. 2017 Jul 19;16(1):125. doi: 10.1186/s12934-017-0732-7.
4
Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering.系统代谢工程策略：系统生物学与合成生物学和代谢工程的整合。
Trends Biotechnol. 2019 Aug;37(8):817-837. doi: 10.1016/j.tibtech.2019.01.003. Epub 2019 Feb 5.
5
Towards the Microbial Production of Plant-Derived Anticancer Drugs.迈向植物源抗癌药物的微生物生产。
Trends Cancer. 2020 Jun;6(6):444-448. doi: 10.1016/j.trecan.2020.02.004. Epub 2020 Mar 5.
6
Synthetic Biology for Specialty Chemicals.用于特种化学品的合成生物学
Annu Rev Chem Biomol Eng. 2015;6:35-52. doi: 10.1146/annurev-chembioeng-061114-123303. Epub 2015 Jan 30.
7
[Improving industrial microbial stress resistance by metabolic engineering: a review].通过代谢工程提高工业微生物抗逆性：综述
Sheng Wu Gong Cheng Xue Bao. 2010 Sep;26(9):1209-17.
8
Recent advances in synthetic biology for engineering isoprenoid production in yeast.酵母中用于工程化生产类异戊二烯的合成生物学最新进展。
Curr Opin Chem Biol. 2017 Oct;40:47-56. doi: 10.1016/j.cbpa.2017.05.017. Epub 2017 Jun 14.
9
Microbial cell factories based on filamentous bacteria, yeasts, and fungi.基于丝状细菌、酵母和真菌的微生物细胞工厂。
Microb Cell Fact. 2023 Jan 30;22(1):20. doi: 10.1186/s12934-023-02025-1.
10
[Biosynthesis of adipic acid].[己二酸的生物合成]
Sheng Wu Gong Cheng Xue Bao. 2013 Oct;29(10):1374-85.
引用本文的文献
1
Role of Enzyme Technologies and Applied Enzymology in Valorising Seaweed Bioproducts.酶技术与应用酶学在海藻生物制品增值中的作用。
Mar Drugs. 2025 Jul 29;23(8):303. doi: 10.3390/md23080303.
2
Upcycling Agricultural Waste for Functional Interfaces: Yellow Onion Skin-Derived Dyes for Cellulosic Materials.将农业废弃物升级转化为功能性界面：用于纤维素材料的黄洋葱皮衍生染料。
ACS Omega. 2025 Aug 11;10(32):36511-36526. doi: 10.1021/acsomega.5c05183. eCollection 2025 Aug 19.
3
Revolutionizing organic synthesis through green chemistry: metal-free, bio-based, and microwave-assisted methods.通过绿色化学革新有机合成：无金属、生物基及微波辅助方法。
Front Chem. 2025 Aug 4;13:1656935. doi: 10.3389/fchem.2025.1656935. eCollection 2025.
4
Synthesis, Characterization, and Preliminary Analysis of Squid Pen Trypsin Hydrolysates and Chitosan Microcapsules.鱿鱼墨囊胰蛋白酶水解产物与壳聚糖微胶囊的合成、表征及初步分析
Int J Mol Sci. 2025 Mar 22;26(7):2885. doi: 10.3390/ijms26072885.
5
Metabolic Engineering of for Efficient Retinol Synthesis.用于高效视黄醇合成的代谢工程。
J Fungi (Basel). 2023 Apr 26;9(5):512. doi: 10.3390/jof9050512.
6
Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.糖苷磷酸化酶的发现和生物技术开发。
Int J Mol Sci. 2022 Mar 11;23(6):3043. doi: 10.3390/ijms23063043.
7
Synthetic biology as driver for the biologization of materials sciences.合成生物学作为材料科学生物化的驱动力。
Mater Today Bio. 2021 May 26;11:100115. doi: 10.1016/j.mtbio.2021.100115. eCollection 2021 Jun.
8
Bacterial Biopolymer: Its Role in Pathogenesis to Effective Biomaterials.细菌生物聚合物：其在发病机制至有效生物材料中的作用。
Polymers (Basel). 2021 Apr 12;13(8):1242. doi: 10.3390/polym13081242.
9
Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection.基于寡核苷酸重组和 Cas9 介导的反筛选的基因组编辑。
ACS Synth Biol. 2020 Jul 17;9(7):1693-1704. doi: 10.1021/acssynbio.0c00022. Epub 2020 Jun 22.
10
Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.微生物生产菌株的快速原型设计，用于潜在材料单体的生物制造。
Metab Eng. 2020 Jul;60:168-182. doi: 10.1016/j.ymben.2020.04.008. Epub 2020 Apr 23.
本文引用的文献
1
Improving designer glycan production in through model-guided metabolic engineering.通过模型引导的代谢工程改善设计聚糖的生产。
Metab Eng Commun. 2019 Mar 29;9:e00088. doi: 10.1016/j.mec.2019.e00088. eCollection 2019 Dec.
2
Hyaluronan production and molecular weight is enhanced in pathway-engineered strains of lactate dehydrogenase-deficient .在乳酸脱氢酶缺陷的途径工程菌株中，透明质酸的产生和分子量增加。
Metab Eng Commun. 2016 Jan 22;3:15-23. doi: 10.1016/j.meteno.2016.01.003. eCollection 2016 Dec.
3
Four-Dimensional Bioprinting As a New Era for Tissue Engineering and Regenerative Medicine.四维生物打印——组织工程与再生医学的新时代
Front Bioeng Biotechnol. 2017 Oct 17;5:61. doi: 10.3389/fbioe.2017.00061. eCollection 2017.
4
Enhanced Biosynthesis of Hyaluronic Acid Using Engineered Corynebacterium glutamicum Via Metabolic Pathway Regulation.利用工程化谷氨酸棒杆菌通过代谢途径调控来增强透明质酸的生物合成。
Biotechnol J. 2017 Oct;12(10). doi: 10.1002/biot.201700191. Epub 2017 Sep 28.
5
Engineered Fluorine Metabolism and Fluoropolymer Production in Living Cells.在活细胞中构建氟代谢和氟聚合物的生产。
Angew Chem Int Ed Engl. 2017 Oct 23;56(44):13637-13640. doi: 10.1002/anie.201706696. Epub 2017 Sep 26.
6
Improved Yield of High Molecular Weight Hyaluronic Acid Production in a Stable Strain of Streptococcus zooepidemicus via the Elimination of the Hyaluronidase-Encoding Gene.通过消除透明质酸酶编码基因提高兽疫链球菌稳定菌株中高分子量透明质酸的产量
Mol Biotechnol. 2017 Jun;59(6):192-199. doi: 10.1007/s12033-017-0005-z.
7
Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit.利用不依赖途径的群体感应电路对工程菌中代谢通量进行动态调控。
Nat Biotechnol. 2017 Mar;35(3):273-279. doi: 10.1038/nbt.3796. Epub 2017 Feb 13.
8
Biocompatible bacterial cellulose membrane in dural defect repair of rat.生物相容性细菌纤维素膜在大鼠硬脑膜缺损修复中的应用
J Mater Sci Mater Med. 2017 Mar;28(3):37. doi: 10.1007/s10856-016-5828-9. Epub 2017 Jan 31.
9
Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library.通过筛选转座子插入文库鉴定影响荧光假单胞菌中藻酸盐生物合成的基因。
BMC Genomics. 2017 Jan 3;18(1):11. doi: 10.1186/s12864-016-3467-7.
10
Genome-wide analyses of chitin synthases identify horizontal gene transfers towards bacteria and allow a robust and unifying classification into fungi.几丁质合成酶的全基因组分析确定了向细菌的水平基因转移，并允许对真菌进行稳健且统一的分类。
BMC Evol Biol. 2016 Nov 24;16(1):252. doi: 10.1186/s12862-016-0815-9.
Zotero 插件