Department of Pathogenesis and Control of Oral Disease, Iwate Medical University School of Dentistry, Morioka, Iwate, Japan.
J Microbiol Methods. 2010 Mar;80(3):313-5. doi: 10.1016/j.mimet.2010.01.015. Epub 2010 Jan 20.
To evaluate enzymes that produce hydrogen sulfide (H(2)S) in species of Fusobacterium nucleatum, we developed an assay based on SDS-polyacrylamide gel electrophoresis with renaturation followed by active staining. This assay provided precise insight into the enzymes that produce H(2)S in terms of their number and molecular weights.
为了评估产硫化氢（H₂S）的梭菌属（Fusobacterium nucleatum）的酶，我们开发了一种基于 SDS-聚丙烯酰胺凝胶电泳的复性后活性染色的检测方法。该方法能够精确地了解产生 H₂S 的酶的数量和分子量。
