Kezuka Yuichiro, Abe Naoto, Yoshida Yasuo, Nonaka Takamasa
Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Iwate 028-3694, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1507-10. doi: 10.1107/S1744309112042546. Epub 2012 Nov 14.
Hydrogen sulfide produced by oral bacteria is responsible for oral malodour. Two homologous hydrogen sulfide-producing enzymes, Fn1220 and Cdl, from Fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. X-ray diffraction data were collected from the crystals using a synchrotron-radiation source. The Fn1220 crystal belonged to tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 Å) and the Cdl crystal belonged to monoclinic space group P2(1) (unit-cell parameters a=84.9, b=70.9, c=87.6 Å, β=90.3°).
口腔细菌产生的硫化氢是导致口腔异味的原因。从具核梭杆菌（一种能活跃产生硫化氢的细菌）中提取了两种同源的硫化氢生成酶Fn1220和Cdl，对其进行了过量表达、纯化和结晶。使用同步辐射源从晶体中收集了X射线衍射数据。Fn1220晶体属于四方晶系空间群P4(1)2(1)2或P4(3)2(1)2（晶胞参数a = b = 116.8，c = 99.2 Å），Cdl晶体属于单斜晶系空间群P2(1)（晶胞参数a = 84.9，b = 70.9，c = 87.6 Å，β = 90.3°）。
