Faculty of Science, University of South Bohemia, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic.
Virus Res. 2010 Apr;149(1):119-23. doi: 10.1016/j.virusres.2010.01.005. Epub 2010 Jan 20.
Tahyna virus (TAHV), a mosquito-borne bunyavirus (California group), is frequently associated with inapparent or influenza-like (Valtice fever) infections in humans, rarely leading to atypical pneumonia or meningitis. Field TAHV strains exhibit a high variability in their biological properties with respect to virulence for laboratory mouse, temperature-sensitivity or character of plaques in cell culture. In consideration of the variations in the antigenic properties TAHV and its potential genetic variability, we analyzed complete nucleotide sequences of the small (S) and medium (M) genomic segments of field TAHV strains with different combinations of phenotypic markers. S segment was highly conservative in all analyzed TAHV strains. Within the M segment, the highest variability was observed in the G(C) gene encoding viral envelope protein and to a less extent also in the NSm gene. However, 5' and 3' non-coding regions of M segment, as well as in G(N) gene exhibited highly conservative pattern, indicating its functional importance, but minor or no role in the determination of biological properties of TAHV field strains.
塔希纳病毒（TAHV）是一种由蚊子传播的布尼亚病毒（加利福尼亚组），常引起人类无症状或类似流感的（瓦尔季采热）感染，很少导致非典型肺炎或脑膜炎。野外 TAHV 株在其对实验室小鼠的毒力、温度敏感性或细胞培养中的蚀斑特征等生物学特性方面表现出高度的变异性。考虑到 TAHV 的抗原特性及其潜在的遗传变异性，我们分析了具有不同表型标记组合的野外 TAHV 株的小（S）和中（M）基因组片段的完整核苷酸序列。在所有分析的 TAHV 株中，S 片段高度保守。在 M 片段内，编码病毒包膜蛋白的 G(C)基因观察到最高的变异性，而 NSm 基因的变异性也较低。然而，M 片段的 5'和 3'非编码区以及 G(N)基因表现出高度保守的模式，表明其功能重要性，但在确定 TAHV 野外株的生物学特性方面作用较小或没有作用。
