酵母乙酰转移酶突变体的表达谱分析确定了基因调控的冗余途径。
Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation.
机构信息
Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089-2910, USA.
出版信息
BMC Genomics. 2010 Jan 22;11:59. doi: 10.1186/1471-2164-11-59.
BACKGROUND
Histone acetyltransferase enzymes (HATs) are implicated in regulation of transcription. HATs from different families may overlap in target and substrate specificity.
RESULTS
We isolated the elp3+ gene encoding the histone acetyltransferase subunit of the Elongator complex in fission yeast and characterized the phenotype of an Deltaelp3 mutant. We examined genetic interactions between Deltaelp3 and two other HAT mutants, Deltamst2 and Deltagcn5 and used whole genome microarray analysis to analyze their effects on gene expression.
CONCLUSIONS
Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions. Consistent with this, overlapping specificity in histone H3 acetylation is observed. However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.
背景
组蛋白乙酰转移酶（HATs）参与转录调控。不同家族的 HATs 在靶标和底物特异性上可能存在重叠。
结果
我们从裂殖酵母中分离出编码延伸复合物组蛋白乙酰转移酶亚基的 elp3+基因，并对Δelp3 突变体的表型进行了表征。我们研究了Δelp3 与另外两个 HAT 突变体 Deltamst2 和 Deltagcn5 之间的遗传相互作用，并使用全基因组微阵列分析来分析它们对基因表达的影响。
结论
在单个、双突变体和三突变体中的表型和表达谱的比较表明，这些 HAT 酶具有重叠的功能。这与组蛋白 H3 乙酰化的特异性重叠一致。然而，没有证据表明与另一种 HAT 酶（由必需的 mst1+基因编码）重叠。
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