Johnsson Anna, Durand-Dubief Mickaël, Xue-Franzén Yongtao, Rönnerblad Michelle, Ekwall Karl, Wright Anthony
Department of Biosciences and Medical Nutrition, Karolinska Institute, S-141 57 Huddinge, Sweden.
EMBO Rep. 2009 Sep;10(9):1009-14. doi: 10.1038/embor.2009.127. Epub 2009 Jul 24.
Histone acetylation and deacetylation are important for gene regulation. The histone acetyltransferase, Gcn5, is an activator of transcriptional initiation that is recruited to gene promoters. Here, we map genome-wide Gcn5 occupancy and histone H3K14ac at high resolution. Gcn5 is predominantly localized to coding regions of highly transcribed genes, where it collaborates antagonistically with the class-II histone deacetylase, Clr3, to modulate H3K14ac levels and transcriptional elongation. An interplay between Gcn5 and Clr3 is crucial for the regulation of many stress-response genes. Our findings suggest a new role for Gcn5 during transcriptional elongation, in addition to its known role in transcriptional initiation.
组蛋白乙酰化和去乙酰化对基因调控至关重要。组蛋白乙酰转移酶Gcn5是一种转录起始激活因子，可被招募至基因启动子区域。在此，我们以高分辨率绘制了全基因组范围内Gcn5的占据情况以及组蛋白H3K14ac。Gcn5主要定位于高转录基因的编码区域，在该区域它与II类组蛋白去乙酰化酶Clr3协同发挥拮抗作用，以调节H3K14ac水平和转录延伸。Gcn5与Clr3之间的相互作用对于许多应激反应基因的调控至关重要。我们的研究结果表明，Gcn5除了在转录起始中发挥已知作用外，在转录延伸过程中也具有新的作用。
