Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
Department of Biology, Systematic Botany and Mycology, Ludwig-Maximilians-Universität München, Munich, Germany.
Virology. 2010 Mar 30;399(1):129-133. doi: 10.1016/j.virol.2009.12.023. Epub 2010 Jan 22.
Warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. Human papillomaviruses (HPV) are considered the causal agents for the development of such warts. We isolated the novel type HPV-117 from a persisting wart by rolling circle amplification. One hundred eighteen warts from immunocompetent patients (IC) and 49 warts from OTR were analyzed by HPV-117 E6 type-specific PCR. As inferred from a phylogenetic analysis, the new type HPV-117 belonged to alpha-PV species 2, including the most similar types HPV-10 and HPV-94. The general prevalence of HPV-117 in warts was 2% in IC (2/118), and 12% in OTR (6/49). The high viral load in dysplastic cells of a Verruca vulgaris was shown by in situ hybridization. Our results suggest an active role of the novel type in the development of cutaneous warts of OTR.
免疫抑制器官移植受者（OTR）的疣持续多年，并且可能进展为非黑色素瘤皮肤癌。人乳头瘤病毒（HPV）被认为是导致此类疣发展的原因。我们通过滚环扩增从持续存在的疣中分离出新型 HPV-117。通过 HPV-117 E6 型特异性 PCR 分析了 118 例免疫功能正常患者（IC）和 49 例 OTR 的疣。从系统发育分析推断，新型 HPV-117 属于α-PV 种 2，包括最相似的 HPV-10 和 HPV-94 型。IC 中的 HPV-117 总流行率为 2%（2/118），OTR 中为 12%（6/49）。原位杂交显示在寻常疣的异型细胞中存在高病毒载量。我们的结果表明，新型 HPV-117 在 OTR 皮肤疣的发展中起积极作用。
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