Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
Department of Biology, Systematic Botany and Mycology, Geo Bio-Center Ludwig-Maximilians-Universität München, Munich, Germany.
J Gen Virol. 2011 Jul;92(Pt 7):1585-1594. doi: 10.1099/vir.0.030593-0. Epub 2011 Apr 6.
Viral warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. The types of human papillomaviruses (HPV) in such lesions are different from that seen in the general population. A subset of these lesions is not infected with the classical wart-associated HPV types. In order to gain a better understanding of the HPV types in those lesions, we isolated ten novel HPVs from persisting keratotic lesions of immunosuppressed OTRs by rolling circle amplification and subsequent long-template PCR. Additionally, we sequenced and characterized the whole genome of the ten novel HPV types. Phylogenetic analyses revealed that nine HPV types belonged to the genus Gammapapillomavirus (γ-PV) and one to the genus Betapapillomavirus. In a phylogenetic analysis using L1 fragments of human and non-human PV types, primate papillomaviruses and our novel HPV types nested within the genus γ-PV in a highly polyphyletic pattern. This study significantly broadens the knowledge concerning the diversity and evolution of the poorly known γ-PV types.
免疫抑制器官移植受者（OTR）的病毒疣持续多年，并可能进展为非黑素瘤皮肤癌。此类病变中的人乳头瘤病毒（HPV）类型与一般人群中所见的不同。这些病变中有一部分并未感染与经典疣相关的 HPV 类型。为了更好地了解这些病变中的 HPV 类型，我们通过滚环扩增和随后的长模板 PCR 从持续角化病变的免疫抑制 OTR 中分离出十种新型 HPV。此外，我们还对这十种新型 HPV 类型的全基因组进行了测序和特征描述。系统发育分析显示，九种 HPV 类型属于γ-乳头瘤病毒属（γ-PV），一种属于β-乳头瘤病毒属。在使用人类和非人类 PV 类型的 L1 片段进行的系统发育分析中，灵长类动物乳头瘤病毒和我们的新型 HPV 类型在一个高度多系的模式中嵌套在γ-PV 属内。这项研究显著扩展了对了解甚少的γ-PV 类型的多样性和进化的认识。
