Zhu Min, Zhou Zhengyu, Chen Yan, Schreiber Ronda, Ransom John T, Fraser John K, Hedrick Marc H, Pinkernell Kai, Kuo Hai-Chien
Department of Regenerative Cell Technology, Cytori Therapeutics, Inc., 3020 Callan Road, San Diego, CA 92121, USA.
Ann Plast Surg. 2010 Feb;64(2):222-8. doi: 10.1097/SAP.0b013e31819ae05c.
Current practice of autologous fat transfer for soft tissue augmentation is limited by poor long-term graft retention. Adipose-derived regenerative cells (ADRCs) contain several types of stem and regenerative cells, which may help improve graft retention through multiple mechanisms. Using a murine fat transplantation model, ADRCs were added to transplanted fat to test whether ADRCs could improve the long-term retention of the grafts. This study showed, at both 6 and 9 months after transplantation, ADRCs not only increased graft retention by 2-fold but also improved the quality of the grafts. ADRC-supplemented grafts had a higher capillary density, indicating ADRCs could promote neovascularization. Further cell tracking and gene expression studies suggest ADRCs may promote angiogenesis and adipocyte differentiation and prevent apoptosis through the expression of various growth factors, including VEGFA and IGF-1. Taken together, these results suggest a potential clinical utility of ADRCs in facilitating autologous fat transfer for soft tissue augmentation.
目前用于软组织填充的自体脂肪移植技术，因长期移植留存率低而受到限制。脂肪来源的再生细胞（ADRCs）包含多种类型的干细胞和再生细胞，可能通过多种机制帮助提高移植留存率。利用小鼠脂肪移植模型，将ADRCs添加到移植脂肪中，以测试ADRCs是否能提高移植脂肪的长期留存率。该研究表明，在移植后6个月和9个月时，ADRCs不仅使移植留存率提高了两倍，还改善了移植脂肪的质量。补充了ADRCs的移植脂肪具有更高的毛细血管密度，表明ADRCs可促进新血管形成。进一步的细胞追踪和基因表达研究表明，ADRCs可能通过包括VEGFA和IGF-1在内的多种生长因子的表达来促进血管生成和脂肪细胞分化，并防止细胞凋亡。综上所述，这些结果表明ADRCs在促进自体脂肪移植用于软组织填充方面具有潜在的临床应用价值。
