Park Choa, Lee Ok-Hee, Park Jin Ju, Yoo Jiyoon, Kwon Euna, Park Jie-Eun, Kang Byeong-Cheol, Lee Dong-Sup, Cho Jaejin
Department of Dental Regenerative Biotechnology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
Front Cell Dev Biol. 2023 Sep 19;11:1219739. doi: 10.3389/fcell.2023.1219739. eCollection 2023.
The clinical application of mesenchymal stem cells (MSCs) is attracting attention due to their excellent safety, convenient acquisition, multipotency, and trophic activity. The clinical effectiveness of transplanted MSCs is well-known in regenerative and immunomodulatory medicine, but there is a demand for their improved viability and regenerative function after transplantation. In this study, we isolated MSCs from adipose tissue from three human donors and generated uniformly sized MSC spheroids (∼100 µm in diameter) called microblocks (MiBs) for dermal reconstitution. The viability and MSC marker expression of MSCs in MiBs were similar to those of monolayer MSCs. Compared with monolayer MSCs, MiBs produced more extracellular matrix (ECM) components, including type I collagen, fibronectin, and hyaluronic acid, and growth factors such as vascular endothelial growth factor and hepatocyte growth factor. Subcutaneously injected MiBs showed skin volume retaining capacity in mice. These results indicate that MiBs could be applied as regenerative medicine for skin conditions such as atrophic scar by having high ECM and bioactive factor expression.
间充质干细胞（MSCs）因其卓越的安全性、便捷的获取方式、多能性和营养活性，其临床应用备受关注。移植的MSCs在再生医学和免疫调节医学中的临床疗效已广为人知，但人们对其移植后提高的存活率和再生功能仍有需求。在本研究中，我们从三名人类供体的脂肪组织中分离出MSCs，并生成了大小均匀的MSC球体（直径约100 µm），称为微块（MiBs），用于皮肤重建。MiBs中MSCs的存活率和MSC标志物表达与单层MSCs相似。与单层MSCs相比，MiBs产生了更多的细胞外基质（ECM）成分，包括I型胶原蛋白、纤连蛋白和透明质酸，以及血管内皮生长因子和肝细胞生长因子等生长因子。皮下注射的MiBs在小鼠中显示出皮肤容积保留能力。这些结果表明，MiBs通过高表达ECM和生物活性因子，可作为萎缩性瘢痕等皮肤疾病的再生医学应用。
