Department of Bioengineering and Biotechnology, Qingdao University of Science and Technology, 266042 Qingdao, China.
Arch Microbiol. 2010 Mar;192(3):229-34. doi: 10.1007/s00203-010-0543-2. Epub 2010 Jan 23.
Agrobacterium sp. II CCBAU 21244 isolated from root nodules of Wisteria sinensis was verified as an endophytic bacterium by inoculation and reisolation tests. However, inoculation with a mixture of this strain and a Sinorhizobium meliloti strain could induce root nodules on W. sinensis and two other woody legumes, which do not form a symbiosis with S. meliloti alone. Rod-shaped and irregular nodules were found on the inoculated plants, in which the S. meliloti strain was detected in all of the nodules; while the Agrobacterium strain was inside of the rod-shaped nodules, or occupied only the nodule surface of the irregular globe-shaped nodules. These findings revealed novel interactions among the symbiotic bacteria, endophytic bacteria and the legume plants, although the mechanisms are still unknown.
从紫藤根瘤中分离出的农杆菌属 II 型 CCBAU 21244 通过接种和再分离试验被证实为内生菌。然而，将该菌株与根瘤菌属 meliloti 菌株混合接种可诱导紫藤和另外两种非豆科木本植物形成根瘤，而这些植物单独与根瘤菌属 meliloti 不能形成共生关系。在接种植物上发现了杆状和不规则形状的根瘤，在所有根瘤中均检测到根瘤菌属 meliloti 菌株；而农杆菌属菌株存在于杆状根瘤内部，或仅占据不规则球形根瘤的表面。尽管机制尚不清楚，但这些发现揭示了共生细菌、内生细菌和豆科植物之间的新相互作用。
