College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University , Yangling, Shaanxi, 712100 , China ; College of Life Sciences, Shangqiu Normal University , Shangqiu, Henan, 476000 , China.
Braz J Microbiol. 2011 Apr;42(2):567-75. doi: 10.1590/S1517-838220110002000022. Epub 2011 Jun 1.
Endophytes MQ23 and MQ23R isolated from Sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. Results showed that MQ23 and MQ23R are potential N2-fixing endophytes and belong to the same species as Bacillus cereus. MQ23 was shown to be able to produce siderophores, IAA, and demonstrate certain antifungal activity to plant pathogenic fungi. Co-inoculation with MQ23+MQ23II showed a more significant effect than inoculation alone in vitro for most of positive actions suggesting they have a cooperative interaction. Results of plant inoculation with endophytes indicated that the growth indexes of co-inoculated MQ23+MQ23II were higher than those of inoculated alone (p<0.05) (the exception being for root fresh weight) when compared to negative control. There have been little of any studies of nonrhizobial putative endophytes with growth-promotion attributes in S. alopecuroides root nodules. This could be exploited as potential bio-inoculants and biocontrol agents in agriculture.
从苦参根瘤中分离出的内生菌 MQ23 和 MQ23R 通过观察其促进植物生长的能力，并采用分子分析技术对其进行了特征描述。结果表明，MQ23 和 MQ23R 是潜在的固氮内生菌，与蜡样芽孢杆菌同属一个物种。结果显示，MQ23 能够产生铁载体、IAA，并对植物病原真菌表现出一定的抑菌活性。与单独接种相比，MQ23+MQ23II 的共接种在体外对大多数阳性作用的效果更为显著，表明它们具有协同作用。内生菌接种植物的结果表明，与阴性对照相比，共接种 MQ23+MQ23II 的生长指标（p<0.05）（除根鲜重外）均高于单独接种。苦参根瘤中具有促生长特性的非根瘤假定内生菌的研究很少。这可以作为农业中潜在的生物接种剂和生物防治剂加以利用。
