Beruto Margherita
Istituto Regionale per la Floricoltura, Sanremo, Italy.
Methods Mol Biol. 2010;589:29-37. doi: 10.1007/978-1-60327-114-1_4.
Ranunculus asiaticus is an important ornamental species mainly cultivated in the countries surrounding the Mediterranean sea. So far, the multiplication of this plant has been mainly carried out by seed and tuberous root division; however, these systems present many drawbacks. Tissue culture is an attractive alternative for accelerated propagation of selected and indexed genotypes. In this chapter, we present a flow chart for the commercial production of Ranunculus clones by using in vitro axillary budding. Although the price of micropropagated plants is higher compared to traditional material (seedlings and tuberous roots from seed populations), we need to consider that micropropagation helps to supply growers with more performant and healthy genotypes, and a better production schedule can be envisaged for cultivation and packaging.
花毛茛是一种重要的观赏植物，主要在地中海周边国家种植。到目前为止，这种植物的繁殖主要通过种子和块根分割进行；然而，这些方法存在许多缺点。组织培养是加速选定和经过索引的基因型繁殖的一种有吸引力的替代方法。在本章中，我们展示了一个通过体外腋芽萌发进行花毛茛无性系商业化生产的流程图。尽管与传统材料（种子群体的幼苗和块根）相比，微繁殖植物的价格更高，但我们需要考虑到微繁殖有助于为种植者提供性能更好、更健康的基因型，并且可以设想出更好的栽培和包装生产计划。
