Department of Structural Biology, Stanford School of Medicine, 299 Campus Drive West, Stanford, California 94305, USA.
Bioconjug Chem. 2010 Feb 17;21(2):214-8. doi: 10.1021/bc900135d.
By adjustment of solvent conditions for synthesis, virtually monodisperse 4-mercaptobenzoic acid (p-MBA) monolayer-protected gold nanoparticles, 2 and 3 nm in diameter, were obtained. Large single crystals of the 2 nm particles could be grown from the reaction mixture. Uniformity was also demonstrated by the formation of two-dimensional arrays and by quantitative high-angle annular dark-field scanning transmission electron microscopy. The 2 and 3 nm particles were spontaneously reactive for conjugation with proteins and DNA, and further reaction could be prevented by repassivation with glutathione. Conjugates with antibody Fc fragment could be used to identify TAP-tagged proteins of interest in electron micrographs, through the binding of a pair of particles to the pair of protein A domains in the TAP tag.
通过调整合成的溶剂条件，可以获得几乎单分散的 4-巯基苯甲酸（p-MBA）单层保护的金纳米粒子，直径为 2nm 和 3nm。可以从反应混合物中生长出 2nm 颗粒的大单晶。通过二维阵列的形成和定量高角度环形暗场扫描透射电子显微镜也证明了其均匀性。2nm 和 3nm 颗粒自发地与蛋白质和 DNA 发生反应，并且可以通过用谷胱甘肽重新钝化来防止进一步的反应。与抗体 Fc 片段的缀合物可以通过一对颗粒与 TAP 标签中的一对蛋白 A 结构域的结合，用于在电子显微镜照片中鉴定感兴趣的 TAP 标记蛋白。
