钙调神经磷酸酶和 Crz1 在光滑念珠菌抗真菌敏感性和毒力中的作用。
Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of Candida glabrata.
机构信息
Department of Molecular Microbiology and Immunology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
出版信息
Antimicrob Agents Chemother. 2010 Apr;54(4):1639-43. doi: 10.1128/AAC.01364-09. Epub 2010 Jan 25.
Abstract
A Candida glabrata calcineurin mutant exhibited increased susceptibility to both azole antifungal and cell wall-damaging agents and was also attenuated in virulence. Although a mutant lacking the downstream transcription factor Crz1 displayed a cell wall-associated phenotype intermediate to that of the calcineurin mutant and was modestly attenuated in virulence, it did not show increased azole susceptibility. These results suggest that calcineurin regulates both Crz1-dependent and -independent pathways depending on the type of stress.
摘要
光滑念珠菌钙调神经磷酸酶突变体对唑类抗真菌药物和细胞壁破坏剂的敏感性均增加，其毒力也减弱。虽然缺乏下游转录因子 Crz1 的突变体表现出与钙调神经磷酸酶突变体相关的细胞壁表型，但毒力中度减弱，对唑类药物的敏感性并未增加。这些结果表明，钙调神经磷酸酶根据应激的类型调节依赖于 Crz1 的和不依赖于 Crz1 的途径。
相似文献
1
Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of Candida glabrata.钙调神经磷酸酶和 Crz1 在光滑念珠菌抗真菌敏感性和毒力中的作用。
Antimicrob Agents Chemother. 2010 Apr;54(4):1639-43. doi: 10.1128/AAC.01364-09. Epub 2010 Jan 25.
2
Vacuolar proton-translocating ATPase is required for antifungal resistance and virulence of Candida glabrata.液泡质子转运 ATP 酶是光滑念珠菌抗真菌耐药性和毒力所必需的。
PLoS One. 2019 Jan 23;14(1):e0210883. doi: 10.1371/journal.pone.0210883. eCollection 2019.
3
The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata.甘露糖磷酸肌醇连接的天冬氨酸蛋白酶 Yps1 在 Candida glabrata 中受钙调神经磷酸酶-Crz1 和 Slt2 MAPK 途径的转录调控。
FEMS Yeast Res. 2011 Aug;11(5):449-56. doi: 10.1111/j.1567-1364.2011.00734.x. Epub 2011 May 16.
4
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata.CgPDR1 调控基因在增强唑类耐药光滑念珠菌毒力中的作用。
PLoS One. 2011 Mar 9;6(3):e17589. doi: 10.1371/journal.pone.0017589.
5
Calcineurin controls hyphal growth, virulence, and drug tolerance of Candida tropicalis.钙调神经磷酸酶控制热带假丝酵母的菌丝生长、毒力和耐药性。
Eukaryot Cell. 2014 Jul;13(7):844-54. doi: 10.1128/EC.00302-13. Epub 2014 Jan 17.
6
Calcineurin is required for Pdr1 transcriptional activation.钙调神经磷酸酶对于 Pdr1 的转录激活是必需的。
mBio. 2023 Dec 19;14(6):e0241623. doi: 10.1128/mbio.02416-23. Epub 2023 Nov 9.
7
CRZ1, a target of the calcineurin pathway in Candida albicans.CRZ1，白色念珠菌中钙调神经磷酸酶途径的一个靶点。
Mol Microbiol. 2006 Mar;59(5):1429-51. doi: 10.1111/j.1365-2958.2005.05037.x.
8
Global analysis of the evolution and mechanism of echinocandin resistance in Candida glabrata.全球分析光滑念珠菌棘白菌素耐药性的进化和机制。
PLoS Pathog. 2012;8(5):e1002718. doi: 10.1371/journal.ppat.1002718. Epub 2012 May 17.
9
Adhesive and biofilm-forming Candida glabrata Lebanese hospital isolates harbour mutations in subtelomeric silencers and adhesins.黎巴嫩医院分离出的具有黏附性和形成生物膜能力的光滑念珠菌在亚端粒沉默子和黏附素中存在突变。
Mycoses. 2024 Jun;67(6):e13750. doi: 10.1111/myc.13750.
10
Role of the Slt2 mitogen-activated protein kinase pathway in cell wall integrity and virulence in Candida glabrata.Slt2 丝裂原活化蛋白激酶途径在光滑念珠菌细胞壁完整性和毒力中的作用。
FEMS Yeast Res. 2010 May;10(3):343-52. doi: 10.1111/j.1567-1364.2010.00611.x. Epub 2010 Feb 3.
引用本文的文献
1
Antifungal drug resistance in Candida glabrata: role of cellular signaling and gene regulatory networks.光滑念珠菌的抗真菌药物耐药性：细胞信号传导和基因调控网络的作用
FEMS Yeast Res. 2025 Jan 30;25. doi: 10.1093/femsyr/foaf025.
2
Mechanisms of multidrug resistance caused by an Ipi1 mutation in the fungal pathogen Candida glabrata.光滑念珠菌中Ipi1突变导致多药耐药的机制
Nat Commun. 2025 Jan 25;16(1):1023. doi: 10.1038/s41467-025-56269-z.
3
mutation in decreases manogepix susceptibility owing to increased expression.由于 表达增加， 中的突变降低了马尼戈匹克的敏感性。 （注：原文中部分关键信息缺失，翻译可能不够完整准确）
Antimicrob Agents Chemother. 2025 Feb 13;69(2):e0150824. doi: 10.1128/aac.01508-24. Epub 2024 Dec 18.
4
Calcineurin-dependent contributions to fitness in the opportunistic pathogen .钙调神经磷酸酶依赖性对机会性病原体适应性的贡献。
mSphere. 2024 Jan 30;9(1):e0055423. doi: 10.1128/msphere.00554-23. Epub 2024 Jan 3.
5
Calcineurin: The Achilles' heel of fungal pathogens.钙调神经磷酸酶：真菌病原体的致命弱点。
PLoS Pathog. 2023 Jul 6;19(7):e1011445. doi: 10.1371/journal.ppat.1011445. eCollection 2023 Jul.
6
Updating Insights into the Regulatory Mechanisms of Calcineurin-Activated Transcription Factor Crz1 in Pathogenic Fungi.深入了解致病真菌中钙调神经磷酸酶激活转录因子Crz1的调控机制的最新进展
J Fungi (Basel). 2022 Oct 14;8(10):1082. doi: 10.3390/jof8101082.
7
Histone Acetylation Regulator Gcn5 Mediates Drug Resistance and Virulence of Candida glabrata.组蛋白乙酰化调控因子 Gcn5 介导光滑念珠菌的耐药性和毒力。
Microbiol Spectr. 2022 Jun 29;10(3):e0096322. doi: 10.1128/spectrum.00963-22. Epub 2022 Jun 6.
8
The effect of antifungal resistance development on the virulence of Candida species.抗真菌耐药性发展对念珠菌属物种毒力的影响。
FEMS Yeast Res. 2022 Apr 26;22(1). doi: 10.1093/femsyr/foac019.
9
CRISPR-Cas9 approach confirms Calcineurin-responsive zinc finger 1 (Crz1) transcription factor as a promising therapeutic target in echinocandin-resistant Candida glabrata.CRISPR-Cas9 方法证实钙调神经磷酸酶反应锌指蛋白 1（Crz1）转录因子是棘白菌素耐药光滑念珠菌有前途的治疗靶点。
PLoS One. 2022 Mar 18;17(3):e0265777. doi: 10.1371/journal.pone.0265777. eCollection 2022.
10
Unmasking of CgYor1-Dependent Azole Resistance Mediated by Target of Rapamycin (TOR) and Calcineurin Signaling in Candida glabrata.棘白菌素耐药性的去掩蔽作用：由雷帕霉素（TOR）和钙调神经磷酸酶信号通路介导的 Candida glabrata 中 CgYor1 依赖性
**解析**：
- Unmasking 这个单词有多重含义，但是在此处为“去掩蔽作用”的意思。
- TOR 为“target of rapamycin”的缩写，意思是雷帕霉素靶蛋白。
- Calcineurin 为“钙调神经磷酸酶”的意思。
mBio. 2022 Feb 22;13(1):e0354521. doi: 10.1128/mbio.03545-21. Epub 2022 Jan 18.
本文引用的文献
1
Calcineurin target CrzA regulates conidial germination, hyphal growth, and pathogenesis of Aspergillus fumigatus.钙调神经磷酸酶靶点CrzA调节烟曲霉的分生孢子萌发、菌丝生长和致病性。
Eukaryot Cell. 2008 Jul;7(7):1085-97. doi: 10.1128/EC.00086-08. Epub 2008 May 2.
2
Functional characterization of the Aspergillus fumigatus CRZ1 homologue, CrzA.烟曲霉CRZ1同源物CrzA的功能表征
Mol Microbiol. 2008 Mar;67(6):1274-91. doi: 10.1111/j.1365-2958.2008.06122.x. Epub 2008 Feb 20.
3
Harnessing calcineurin as a novel anti-infective agent against invasive fungal infections.利用钙调神经磷酸酶作为一种新型抗侵袭性真菌感染的抗感染药物。
Nat Rev Microbiol. 2007 Jun;5(6):418-30. doi: 10.1038/nrmicro1680.
4
Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red.使用基于荧光增白剂和刚果红的药敏试验鉴定真菌细胞壁突变体。
Nat Protoc. 2006;1(5):2253-6. doi: 10.1038/nprot.2006.397.
5
Epidemiology of invasive candidiasis: a persistent public health problem.侵袭性念珠菌病的流行病学：一个持续存在的公共卫生问题。
Clin Microbiol Rev. 2007 Jan;20(1):133-63. doi: 10.1128/CMR.00029-06.
6
The bZip transcription factor Cgap1p is involved in multidrug resistance and required for activation of multidrug transporter gene CgFLR1 in Candida glabrata.bZip转录因子Cgap1p参与多药耐药，并且是光滑念珠菌中多药转运蛋白基因CgFLR1激活所必需的。
Gene. 2007 Jan 15;386(1-2):63-72. doi: 10.1016/j.gene.2006.08.010. Epub 2006 Aug 24.
7
Functional characterization of the Aspergillus fumigatus calcineurin.烟曲霉钙调神经磷酸酶的功能特性
Fungal Genet Biol. 2007 Mar;44(3):219-30. doi: 10.1016/j.fgb.2006.08.004. Epub 2006 Sep 20.
8
Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus.钙调神经磷酸酶调控烟曲霉的生长、形态及致病性。
Eukaryot Cell. 2006 Jul;5(7):1091-103. doi: 10.1128/EC.00139-06.
9
Role of calcineurin in stress resistance, morphogenesis, and virulence of a Candida albicans wild-type strain.钙调神经磷酸酶在白色念珠菌野生型菌株的应激抗性、形态发生及毒力中的作用
Infect Immun. 2006 Jul;74(7):4366-9. doi: 10.1128/IAI.00142-06.
10
Kre29p is a novel nuclear protein involved in DNA repair and mitotic fidelity in Candida glabrata.Kre29p是一种新型核蛋白，参与光滑念珠菌的DNA修复和有丝分裂保真度。
Curr Genet. 2006 Jul;50(1):11-22. doi: 10.1007/s00294-006-0072-3. Epub 2006 Apr 28.
Zotero 插件