低能量氦氖激光照射对Wistar大鼠切除性糖尿病伤口愈合动力学的光刺激作用
Photo-stimulatory effect of low energy helium-neon laser irradiation on excisional diabetic wound healing dynamics in Wistar rats.
作者信息
Maiya Arun G, Kumar Pramod, Nayak Shivanand
机构信息
Department of Physiotherapy, Manipal Medical College, Manipal, India.
出版信息
Indian J Dermatol. 2009;54(4):323-9. doi: 10.4103/0019-5154.57606.
Abstract
BACKGROUND
Generally, the significances of laser photo stimulation are now accepted, but the laser light facilitates wound healing and tissue repair remains poorly understood.
AIMS
We have examined the hypothesis that the laser photo stimulation can enhance the collagen production in diabetic wounds using the excision wound model in the Wistar rat model.
METHODS
The circular wounds were created on the dorsum of the back of the animals. The animals were divided into two groups. The study group (N = 24) wound was treated with 632.8 nm He-Ne laser at a dose of 3-9 J/cm(2) for 5 days a week until the wounds healed completely. The control group was sham irradiated.
RESULT
A significant increase in the hydroxyproline content and reduction in the wound size were observed in the study group. The pro-healing actions seem to be due to increased collagen deposition as well as better alignment and maturation.
CONCLUSION
The biochemical analysis and clinical observation suggested that 3-6 J/cm(2) laser photo stimulation facilitates the tissue repair process by accelerating collagen production in diabetic wound healing.
摘要
背景
一般来说，激光光刺激的意义目前已被认可，但激光促进伤口愈合和组织修复的机制仍知之甚少。
目的
我们使用Wistar大鼠模型中的切除伤口模型，检验了激光光刺激可增强糖尿病伤口胶原蛋白生成的假说。
方法
在动物背部创建圆形伤口。将动物分为两组。研究组（N = 24）的伤口每周用剂量为3 - 9 J/cm²的632.8 nm氦氖激光治疗5天，直至伤口完全愈合。对照组进行假照射。
结果
研究组中羟脯氨酸含量显著增加，伤口大小减小。促进愈合的作用似乎是由于胶原蛋白沉积增加以及排列和成熟度更好。
结论
生化分析和临床观察表明，3 - 6 J/cm²的激光光刺激通过加速糖尿病伤口愈合中的胶原蛋白生成来促进组织修复过程。
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