Martin B M, Gimbrone M A, Unanue E R, Cotran R S
J Immunol. 1981 Apr;126(4):1510-5.
Cultured mouse peritoneal macrophages secrete a growth-promoting activity that stimulates 3 types of nonlymphoid mesenchymal cells in vitro: fibroblasts, vascular smooth muscle, and vascular endothelium. Production of this macrophage-derived growth factor (MDGF) is directly related to the number of viable macrophages and their time in culture, and is independent of platelet- or plasma-derived serum growth factors. Treatment of cultured macrophages with latex, bacterial lipopolysaccharide, or phorbol myristate acetate results in increased growth factor activity. Preliminary biochemical characterization of MDGF indicates that it is a heat labile (100 degrees C, 2 min), non-dialyzable protein, which contains at least 1 essential disulfide bond. Growth-promoting activity is not adsorbed by CM-Sephadex chromatography, under conditions that effectively remove platelet-derived growth factor(s). Serine protease activity is not required for the action of MDGF. Secretion of macrophage-derived growth factor may be relevant to the function of mononuclear phagocytes in several pathologic processes, including the neovascularization and fibroplasia of wound healing, smooth muscle hyperplasia in atherosclerosis, and proliferative glomerulonephritis.
培养的小鼠腹腔巨噬细胞分泌一种促生长活性物质，该物质在体外可刺激3种非淋巴间充质细胞：成纤维细胞、血管平滑肌细胞和血管内皮细胞。这种巨噬细胞衍生生长因子（MDGF）的产生与存活巨噬细胞的数量及其培养时间直接相关，且独立于血小板或血浆衍生的血清生长因子。用乳胶、细菌脂多糖或佛波酯处理培养的巨噬细胞会导致生长因子活性增加。MDGF 的初步生化特性表明，它是一种热不稳定（100℃，2分钟）、不可透析的蛋白质，含有至少1个必需的二硫键。在有效去除血小板衍生生长因子的条件下，促生长活性不被CM-葡聚糖凝胶色谱吸附。MDGF 的作用不需要丝氨酸蛋白酶活性。巨噬细胞衍生生长因子的分泌可能与单核吞噬细胞在多种病理过程中的功能有关，包括伤口愈合中的新血管形成和纤维增生、动脉粥样硬化中的平滑肌增生以及增殖性肾小球肾炎。
