Allendorf J D, Bessler M, Huang J, Kayton M L, Laird D, Nowygrod R, Treat M R
Columbia University, New York, New York, USA.
Lasers Surg Med. 1997;20(3):340-5. doi: 10.1002/(sici)1096-9101(1997)20:3<340::aid-lsm13>3.0.co;2-h.
Reports in the literature indicate that low energy laser irradiation has a biostimulatory effect on wound healing; however, no mechanism of this effect has been elucidated.
STUDY DESIGN/MATERIALS AND METHODS: We attempted to establish a model from which to study the mechanism of biostimulation. The effects of low energy helium-neon irradiation on wound healing were observed in two rat models. In the first model, 1.5 cm diameter full thickness excisional skin defects were created in the dorsal midline of rats (n = 32). All animals were anesthetized and all eschars were debrided daily. Wound area was determined by caliper measurements for 2 weeks postoperatively. Rats that received a treatment of 1 J/cm2 had two defects in the dorsal skin. One wound was treated and the second was used as its own control. These measurements were not blinded. Rats that received 2 J/cm2, 4 J/cm2, or anesthesia alone had one defect on the dorsal skin. Caliper measurements of these wounds were blinded. We were unable to demonstrate any difference in the rate of wound contracture in rats that received a daily dose of 1 J/cm2, 2 J/cm2, 4 J/cm2, or anesthesia alone (P > 0.8 by student's t-test). In the second model, a single 2 cm longitudinal full thickness skin incision was created in the dorsal midline of each rat (n = 24). No difference was found between rats that received anesthesia alone and those treated daily with 2 J/cm2 as assessed by tensile strength measurements on postoperative days 7 and 14 (P > 0.8 by student's t-test between groups at both time points). These determinations were blinded.
Despite our intentions of studying the mechanism of low energy HeNe biostimulation, we were unable to demonstrate a beneficial effect.
In this study, helium-neon laser irradiation produced no measurable benefit on wound healing.
文献报道表明，低能量激光照射对伤口愈合具有生物刺激作用；然而，这种作用的机制尚未阐明。
研究设计/材料与方法：我们试图建立一个用于研究生物刺激机制的模型。在两种大鼠模型中观察了低能量氦氖激光照射对伤口愈合的影响。在第一个模型中，在大鼠背部中线制造直径为1.5厘米的全层切除性皮肤缺损（n = 32）。所有动物均麻醉，每天清除所有焦痂。术后2周通过卡尺测量确定伤口面积。接受1 J/cm²治疗的大鼠背部皮肤有两个缺损。一个伤口接受治疗，另一个作为自身对照。这些测量未设盲。接受2 J/cm²、4 J/cm²或仅接受麻醉的大鼠背部皮肤有一个缺损。这些伤口的卡尺测量设盲。我们未能证明每天接受1 J/cm²、2 J/cm²、4 J/cm²或仅接受麻醉的大鼠在伤口收缩率上有任何差异（学生t检验，P > 0.8）。在第二个模型中，在每只大鼠的背部中线做一个2厘米长的全层皮肤纵切口（n = 24）。在术后第7天和第14天通过拉伸强度测量评估，发现仅接受麻醉的大鼠与每天接受2 J/cm²治疗的大鼠之间没有差异（两个时间点组间学生t检验，P > 0.8）。这些测定设盲。
尽管我们旨在研究低能量氦氖生物刺激的机制，但我们未能证明其有益效果。
在本研究中，氦氖激光照射对伤口愈合未产生可测量的益处。
