Touitou Yvan, Auzéby André, Camus Françoise, Djeridane Yasmina
Faculté de Médecine Pierre et Marie Curie, Service de Biochimie Médicale et Biologie Moléculaire and INSERM UMRS 975, Paris, France.
J Pediatr Endocrinol Metab. 2009 Nov;22(11):1009-15. doi: 10.1515/jpem.2009.22.11.1009.
The aim of this study was to assess the circadian hormonal profile of two circadian markers, melatonin and cortisol, as well as other steroids in prepubertal boys (Tanner stage I). Nine volunteer healthy prepubertal boys aged 10.8 +/- 0.11 years participated in this study. Concentrations of daily salivary and urinary hormones were quantified around 24-hours, every 3 hours, in daytime samples (collected between 07.00 h +/- 30 min and 21.00 h +/- 30 min) and night-time samples (collected between 21.00 h +/- 30 min and 07.00 h +/- 30 min). Significant differences (p < 0.01) were found between day- and nighttime secretion of salivary melatonin and urinary 6-sulphatoxymelatonin, whereas no significant differences were found between day- and nighttime secretion of salivary and urinary cortisol nor between day- and nighttime secretion of 17-hydroxycorticosteroids (17-OHCS). The circadian profiles of salivary melatonin and cortisol showed large amplitude with a peak occurring at night (approximately 03.00 h) for melatonin and in the early morning (between 06.00 and 09.00 h) for cortisol. The curve patterns of the urinary 6-sulphatoxymelatonin and steroids (free cortisol and 17-OHCS) were coherent with data on saliva. The pattern of salivary androstenedione and testosterone were undetectable due to the very low concentrations of these steroids in the saliva of the prepubertal children. A strong significant positive correlation was observed between the daily salivary melatonin levels and the daily urinary 6-sulphatoxymelatonin excretion (R = 0.968, p < 0.001), and between free urinary cortisol and urinary 17-OHCS (R = 0.733, p = 0.025). The salivary and urinary hormones studied were independent of body mass index. This study shows the relevance of salivary cortisol and melatonin, although lower than in plasma, in testing adrenal and pineal function as markers of circadian rhythms. The data are of interest for the diagnosis and treatment of chronobiological disorders in prepubertal children.
本研究的目的是评估青春期前男孩（坦纳I期）两种昼夜节律标志物褪黑素和皮质醇以及其他类固醇的昼夜激素谱。9名年龄为10.8±0.11岁的健康青春期前男孩志愿者参与了本研究。在白天样本（07:00±30分钟至21:00±30分钟之间采集）和夜间样本（21:00±30分钟至07:00±30分钟之间采集）中，每3小时对每日唾液和尿液中的激素浓度进行24小时定量。唾液褪黑素和尿6-硫酸氧褪黑素的白天和夜间分泌之间存在显著差异（p<0.01），而唾液和尿液皮质醇的白天和夜间分泌之间以及17-羟皮质类固醇（17-OHCS）的白天和夜间分泌之间均未发现显著差异。唾液褪黑素和皮质醇的昼夜谱显示出较大的幅度，褪黑素在夜间（约03:00 h）出现峰值，皮质醇在清晨（06:00至09:00 h之间）出现峰值。尿6-硫酸氧褪黑素和类固醇（游离皮质醇和17-OHCS）的曲线模式与唾液数据一致。由于青春期前儿童唾液中这些类固醇的浓度非常低，唾液雄烯二酮和睾酮的模式无法检测到。每日唾液褪黑素水平与每日尿6-硫酸氧褪黑素排泄之间（R = 0.968，p<0.0）以及游离尿皮质醇与尿17-OHCS之间（R = 0.733，p = 0.025）观察到强显著正相关。所研究的唾液和尿液激素与体重指数无关。本研究表明，尽管唾液皮质醇和褪黑素低于血浆中的水平，但在检测肾上腺和松果体功能作为昼夜节律标志物方面具有相关性。这些数据对于青春期前儿童生物钟紊乱的诊断和治疗具有重要意义。
