School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
J Environ Sci (China). 2009;21(11):1486-90. doi: 10.1016/s1001-0742(08)62444-4.
The laccase-catalyzed conversion of bisphenol A (BPA) in aqueous solutions was studied in the absence and presence of nonionic surfactant Triton X-100. It was found that the addition of Triton X-100 into the reaction system increased the conversion of BPA, especially near the critical micelle concentration of Triton X-100. Also it was found that the stability of laccase was greatly improved in the presence of TritonX-100. Studies on the endogenous fluorescence emission of laccase indicated that there existed an interaction between Triton X-100 and laccase, which was beneficial to folding and stabilizating of laccase. The binding of Triton X-100 to the laccase surface also mitigated the inactivation effect caused by the free radicals and polymerization products. Under otherwise identical conditions, a lower dosage of laccase was needed for the higher conversion of BPA in the presence of Triton X-100.
在水溶液中，研究了漆酶催化双酚 A（BPA）的转化，考察了非离子表面活性剂 Triton X-100 的存在与否。结果表明，在反应体系中添加 Triton X-100 会增加 BPA 的转化率，尤其是在 Triton X-100 的临界胶束浓度附近。还发现，Triton X-100 的存在极大地提高了漆酶的稳定性。对漆酶内源性荧光发射的研究表明，Triton X-100 与漆酶之间存在相互作用，这有利于漆酶的折叠和稳定。Triton X-100 与漆酶表面的结合也减轻了自由基和聚合产物引起的失活效应。在其他相同条件下，在 Triton X-100 存在下，BPA 的转化率更高，所需的漆酶用量更低。
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