Centre de Recherches sur les Macromolecules Vegetales-CNRS, BP53, 38041 Grenoble cedex 9, France.
Biomacromolecules. 2010 Mar 8;11(3):713-20. doi: 10.1021/bm9012937.
Polyelectrolyte microcapsules were prepared by the layer-by-layer assembly of hyaluronic acid (HA) and a polycationic polymer, poly(allylamine) (PAH) or poly(lysine) (PLL). The influence of the polycationic partner on the morphology, stability, permeability properties, and enzymatic degradation of microcapsules was thoroughly analyzed. It was found that these properties could be tuned by shell cross-linking. Confocal microscopy studies of cellular uptake of the capsules showed that the polyelectrolyte shells remain stable outside the cells but readily break open once internalized by cells, suggesting their potential as carrier for intracellular drug delivery.
聚电解质微胶囊是通过透明质酸 (HA) 和聚阳离子聚合物，如聚烯丙胺 (PAH) 或聚赖氨酸 (PLL) 的层层组装制备的。本文深入分析了聚阳离子配体对微胶囊形态、稳定性、渗透性和酶降解性能的影响。结果表明，通过壳交联可以调节这些性质。细胞摄取胶囊的共焦显微镜研究表明，聚电解质壳在细胞外保持稳定，但一旦被细胞内化，就会轻易破裂，这表明它们有可能作为细胞内药物递送的载体。
