Department of Veterinary Public Health and Animal Pathology, Division of Veterinary Pathology, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra, Bologna, Italy.
BMC Vet Res. 2010 Jan 28;6:5. doi: 10.1186/1746-6148-6-5.
Human breast cancer is classified by gene expression profile into subtypes consisting of two hormone (oestrogen and/or progesterone) receptor-positive types (luminal-like A and luminal-like B) and three hormone receptor-negative types [human epidermal growth factor receptor 2-expressing, basal-like, and unclassified ("normal-like")]. Immunohistochemical surrogate panels are also proposed to potentially identify the molecular-based groups. The present study aimed to apply an immunohistochemical panel (anti-ER, -PR, -ERB-B2, -CK 5/6 and -CK14) in a series of canine malignant mammary tumours to verify the molecular-based classification, its correlation with invasion and grade, and its use as a prognostic aid in veterinary practice.
Thirty-five tumours with luminal pattern (ER+ and PR+) were subgrouped into 13 A type and 22 B type, if ERB-B2 positive or negative. Most luminal-like A and basal-like tumours were grade 1 carcinomas, while the percentage of luminal B tumours was higher in grades 2 and 3 (Pearson Chi-square P = 0.009). No difference in the percentage of molecular subtypes was found between simple and complex/mixed carcinomas (Pearson Chi-square P = 0.47). No significant results were obtained by survival analysis, even if basal-like tumours had a more favourable prognosis than luminal-like lesions.
The panel of antibodies identified only three tumour groups (luminal-like A and B, and basal-like) in the dog. Even though canine mammary tumours may be a model of human breast cancer, the existence of the same carcinoma molecular subtypes in women awaits confirmation. Canine mammary carcinomas show high molecular heterogeneity, which would benefit from a classification based on molecular differences. Stage and grade showed independent associations with survival in the multivariate regression, while molecular subtype grouping and histological type did not show associations. This suggests that caution should be used when applying this classification to the dog, in which invasion and grade supply the most important prognostic information.
人类乳腺癌根据基因表达谱分为两种激素（雌激素和/或孕激素）受体阳性型（腔A型和腔 B 型）和三种激素受体阴性型[人表皮生长因子受体 2 表达型、基底样型和未分类型（“正常样”）]。也提出了免疫组织化学替代物来潜在地识别基于分子的群体。本研究旨在应用免疫组织化学面板（抗 ER、PR、ERB-B2、CK5/6 和 CK14）对一系列犬恶性乳腺肿瘤进行检测，以验证基于分子的分类、其与浸润和分级的相关性，并将其作为兽医实践中的预后辅助手段。
35 个具有腔型（ER+和 PR+）的肿瘤分为 13 个 A 型和 22 个 B 型，如果 ERB-B2 阳性或阴性。大多数腔 A 型和基底样肿瘤为 1 级癌，而腔 B 型肿瘤的 2 级和 3 级比例较高（皮尔逊卡方 P = 0.009）。简单型和复杂/混合型癌之间的分子亚型比例无差异（皮尔逊卡方 P = 0.47）。即使基底样肿瘤的预后比腔样病变更有利，生存分析也没有得到显著结果。
抗体面板仅在犬中鉴定出三种肿瘤组（腔 A 和 B 型和基底样型）。尽管犬乳腺肿瘤可能是人类乳腺癌的模型，但女性中是否存在相同的癌分子亚型仍有待证实。犬乳腺肿瘤表现出高度的分子异质性，基于分子差异的分类将受益于此。在多变量回归中，分期和分级与生存有独立关联，而分子亚型分组和组织学类型没有关联。这表明在犬中应用这种分类时应谨慎，其中浸润和分级提供最重要的预后信息。
