Otolaryngology, Department of Surgery, University of Alabama-Birmingham, Birmingham, Alabama, USA.
Am J Rhinol Allergy. 2010 Jan-Feb;24(1):6-10. doi: 10.2500/ajra.2010.24.3379.
Dynamic regulation of respiratory ciliary beat frequency (CBF) is regulated by fluxes in intracellular calcium (Ca(2+)). P2X receptors (P2XR) are extracellular ATP-gated, Ca(2+)-permeable, nonselective cation channels. Zinc increases intracellular Ca(2+) in a sodium (Na(+))-free environment through activation of P2XR channels. We hypothesize that topical zinc increases CBF in a Ca(2+)-dependent fashion as a result of this mechanism.
The apical surface of mouse sinonasal air-liquid interface cultures were bathed in zinc in a Na(+)-free solution with or without Ca(2+). High-speed digital video imaging captured and analyzed CBF at a sampling rate of 100 frames/s.
CBF significantly increased fourfold over baseline from 5.99 +/- 3.16 Hz to 22.4 +/- 4.33 Hz in the presence of zinc chloride (50 micromoles) and calcium chloride (3 mM). This effect is abolished in the presence of extracellular Na(+) and was pH dependent.
Zinc stimulates CBF in the presence of Ca(2+) likely through activation of P2X receptors. Thus, zinc represents a promising agent for stimulation of mucociliary clearance.
呼吸纤毛的拍动频率（CBF）的动态调节受细胞内钙离子（Ca(2+)）的流动调节。P2X 受体（P2XR）是细胞外 ATP 门控、Ca(2+)通透、非选择性阳离子通道。锌在无钠（Na(+)）环境中通过激活 P2XR 通道增加细胞内 Ca(2+)。我们假设这种机制导致局部锌以 Ca(2+)依赖的方式增加 CBF。
在无 Na(+)、有或无 Ca(2+)的溶液中，用锌盐处理鼠鼻气道-液界面培养物的顶膜表面。高速数字视频成像以 100 帧/秒的采样率捕获和分析 CBF。
锌（50 微摩尔）和氯化钙（3 mM）存在时，CBF 从基础水平的 5.99 +/- 3.16 Hz 显著增加四倍至 22.4 +/- 4.33 Hz。这种作用在存在细胞外 Na(+)时被消除，并且依赖于 pH 值。
锌在 Ca(2+)存在下刺激 CBF，可能通过激活 P2X 受体。因此，锌代表一种有前途的刺激黏液纤毛清除的药物。
