从南极洲分离并鉴定一株柴油降解假单胞菌菌株。

Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica.

作者信息

Shukor M Y, Hassan N A A, Jusoh A Z, Perumal N, Shamaan N A, MacCormack W P, Syed M A

机构信息

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.

出版信息

J Environ Biol. 2009 Jan;30(1):1-6.

PMID:20112855

Abstract

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16S rDNA molecular phylogeny and Biolog GN microplate panels and Microlog database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15 degrees C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.

摘要

已从南极洲分离出一种能降解柴油的细菌。基于部分16S rDNA分子系统发育以及Biolog GN微孔板和Microlog数据库，该分离菌株初步鉴定为假单胞菌属菌株DRYJ3。硫酸铵、硝酸盐和亚硝酸盐最有利于该菌株在柴油上生长。该细菌在10至15摄氏度、pH值7.0以及3.5%（v/v）柴油的条件下生长最佳。在培养的第二天至第六天，该菌株对柴油的生物降解效率从1.4%提高到18.8%，在第八天趋于平稳。在粗酶制剂中检测到正构烷烃氧化和醛还原酶活性，表明该细菌存在末端正构烷烃氧化活性。