Research and Development Division, H-Plus Eco Ltd., BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
World J Microbiol Biotechnol. 2012 May;28(5):2057-67. doi: 10.1007/s11274-012-1008-3. Epub 2012 Feb 7.
The diesel-degrading strains, designated as MJ01 and MJ4, were isolated from oil-contaminated soil in Daejeon (South Korea) and were taxonomically characterized using a polyphasic approach and their diesel oil degradation abilities were analyzed. The isolates MJ01 and MJ4 were identified as Acinetobacter haemolyticus and Acinetobacter johnsonii, respectively, based on their 16S rDNA gene sequences, DNA-DNA relatedness, fatty acid profiles and various physiological characteristics. Strains MJ01 and MJ4 were able to use diesel oil as the sole carbon and energy source. Both strains could degrade over 90% of diesel oil with an initial concentration of 20,000 mg/l after incubation for 7 days, the most significant degradation occurred during the first 3 days. To our knowledge, this is the first report on diesel oil-degrading microorganisms among bacterial strains belonging to A. haemolyticus and A. johnsonii.
这两种降解柴油的菌株被命名为 MJ01 和 MJ4，它们是从大田（韩国）受污染的土壤中分离出来的，并通过多相分类法对其进行了分类鉴定，同时还分析了它们的柴油降解能力。根据 16S rDNA 基因序列、DNA-DNA 同源性、脂肪酸图谱和各种生理特性，将分离株 MJ01 和 MJ4 鉴定为解血阿克曼菌和约翰逊不动杆菌。MJ01 和 MJ4 菌株能够利用柴油作为唯一的碳源和能源。在 7 天的培养时间内，两种菌株在初始浓度为 20000mg/L 的柴油中能够降解超过 90%的柴油，其中最显著的降解发生在最初的 3 天内。据我们所知，这是关于属于解血阿克曼菌和约翰逊不动杆菌的细菌菌株降解柴油的首次报道。
