Shukor M Y, Gusmanizar N, Ramli J, Shamaan N A, MacCormack W P, Syed M A
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):107-12.
The presence of acrylamide in the environment poses a threat due to its well known neurotoxic, carcinogenic and teratogenic properties. Human activities in various geographical areas are the main anthropogenic source of acrylamide pollution. In this work, an acrylamide-degrading bacterium was isolated from Antarctic soil. The physiological characteristics and optimum growth conditions of the acrylamide-degrading bacteria were investigated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ7 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. The results showed that the best carbon sources for growth was glucose and sucrose with no significant difference in terms of cellular growth between the two carbon sources (p>0.05). This was followed by fructose and maltose with fructose giving significantly higher cellular growth compared to maltose (p<0.05). Lactose and citric acid did not support growth. The optimum acrylamide concentration as a nitrogen source for cellular growth was at 500 mgl(-1). At this concentration, bacterial growth showed a 2-day lag phase before degradation took place concomitant with an increase in cellular growth. The isolate exhibited optimum growth in between pH 7.5 and 8.5. The effect of incubation temperature on the growth of this isolate showed an optimum growth at 15 degrees C. The characteristics of this isolate suggest that it would be useful in the bioremediation of acrylamide.
环境中丙烯酰胺的存在因其众所周知的神经毒性、致癌性和致畸性而构成威胁。不同地理区域的人类活动是丙烯酰胺污染的主要人为来源。在这项工作中，从南极土壤中分离出了一种丙烯酰胺降解细菌。对该丙烯酰胺降解细菌的生理特性和最佳生长条件进行了研究。基于使用Biolog GN平板的碳利用图谱和部分16S rDNA分子系统发育，该分离株初步鉴定为假单胞菌属菌株DRYJ7。结果表明，最佳生长碳源是葡萄糖和蔗糖，两种碳源之间的细胞生长无显著差异（p>0.05）。其次是果糖和麦芽糖，果糖的细胞生长明显高于麦芽糖（p<0.05）。乳糖和柠檬酸不支持生长。作为细胞生长氮源的最佳丙烯酰胺浓度为500 mg l(-1)。在此浓度下，细菌生长在降解发生之前有一个2天的滞后期，同时细胞生长增加。该分离株在pH 7.5至8.5之间表现出最佳生长。培养温度对该分离株生长的影响表明，在15℃时生长最佳。该分离株的特性表明它可用于丙烯酰胺的生物修复。
