Shukor M Y, Gusmanizar N, Azmi N A, Hamid M, Ramli J, Shamaan N A, Syed M A
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):57-64.
Several local acrylamide-degrading bacteria have been isolated. One of the isolate that exhibited the highest growth on acrylamide as a nitrogen source was then further characterized. The isolate was tentatively identified as Bacillus cereus strain DRY135 based on carbon utilization profiles using Biolog GP plates and partial 16S rDNA molecular phylogeny. The isolate grew optimally in between the temperatures of 25 and 30 degrees C and within the pH range of 6.8 to 7.0. Glucose, fructose, lactose, maltose, mannitol, citric acid and sucrose supported growth with glucose being the best carbon source. Different concentrations of acrylamide ranging from 100 to 4000 mg l(-1) incorporated into the growth media shows that the highest growth was obtained at acrylamide concentrations of between 500 to 1500 mg l(-1). At 1000 mg l(-1) of acrylamide, degradation was 90% completed after ten days of incubation with concomitant cell growth. The metabolite acrylic acid was detected in the media during degradation. Other amides such as methacrylamide, nicotinamide, acetamide, propionamide and urea supported growth with the highest growth supported by acetamide, propionamide and urea. Strain DRY135, however was not able to assimilate 2-chloroacetamide. The characteristics of this isolate suggest that it would be useful in the bioremediation of acrylamide.
已分离出几种本地丙烯酰胺降解细菌。然后对其中一种以丙烯酰胺作为氮源生长最好的分离菌株进行了进一步鉴定。根据使用Biolog GP平板的碳利用谱和部分16S rDNA分子系统发育，该分离菌株初步鉴定为蜡样芽孢杆菌DRY135菌株。该分离菌株在25至30摄氏度的温度范围内以及6.8至7.0的pH范围内生长最佳。葡萄糖、果糖、乳糖、麦芽糖、甘露醇、柠檬酸和蔗糖支持其生长，其中葡萄糖是最佳碳源。在生长培养基中加入浓度范围为100至4000 mg l(-1)的不同浓度丙烯酰胺，结果表明在丙烯酰胺浓度为500至1500 mg l(-1)时生长最高。在1000 mg l(-1)的丙烯酰胺浓度下，培养十天后降解完成90%，同时细胞生长。降解过程中在培养基中检测到代谢产物丙烯酸。其他酰胺如甲基丙烯酰胺、烟酰胺、乙酰胺、丙酰胺和尿素支持其生长，其中乙酰胺、丙酰胺和尿素支持的生长最高。然而，DRY135菌株不能同化2-氯乙酰胺。该分离菌株的特性表明它在丙烯酰胺的生物修复中可能有用。
