Shukor M Y, Husin W S W, Rahman M F A, Shamaan N A, Syed M A
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):129-34.
Sodium dodecyl sulfate (SDS) is one of the main components in the detergent and cosmetic industries. Its bioremediation by suitable microorganism has begun to receive greater attention as the amount of SDS usage increases to a point where treatment plants would not be able to cope with the increasing amount of SDS in wastewater. The purpose of this work was to isolate local SDS-degrading bacteria. Screening was carried out by the conventional enrichment-culture technique. Six SDS-degrading bacteria were isolated. Of these isolates, isolate S14 showed the highest degradation of SDS with 90% degradation after three days of incubation. Isolate S14 was tentatively identified as Klebsiella oxytoca strain DRY14 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. SDS degradation by the bacterium was optimum at 37 degrees 0. Ammonium sulphate; at 2.0 g l(-1), was found to be the best nitrogen source for the growth of strain DRY14. Maximum growth on SDS was observed at pH 7.25. The strain exhibited optimum growth at SDS concentration of 2.0 g l(-1) and was completely inhibited at 10 g l(-1) SDS. At the tolerable initial concentration of 2.0 g l(-1), almost 80% of 2.0 g l(-1) SDS was degraded after 4 days of incubation concomitant with increase in cellular growth. The K(m(app) and V(max(app)) values calculated for the alkylsulfatase from this bacterium were 0.1 mM SDS and 1.07 micromol min(-1) mg(-1) protein, respectively.
十二烷基硫酸钠（SDS）是洗涤剂和化妆品行业的主要成分之一。随着SDS使用量增加到污水处理厂无法应对废水中不断增加的SDS量的程度，利用合适的微生物对其进行生物修复已开始受到更多关注。这项工作的目的是分离本地的SDS降解细菌。通过传统的富集培养技术进行筛选。分离出了6株SDS降解细菌。在这些分离株中，分离株S14在培养三天后对SDS的降解率最高，达到90%。基于使用Biolog GN平板的碳利用图谱和部分16S rDNA分子系统发育分析，分离株S14初步鉴定为产酸克雷伯菌菌株DRY14。该细菌对SDS的降解在37摄氏度时最佳。硫酸铵；浓度为2.0 g l(-1)时，被发现是菌株DRY14生长的最佳氮源。在pH 7.25时观察到在SDS上的最大生长。该菌株在SDS浓度为2.0 g l(-1)时表现出最佳生长，在10 g l(-1) SDS时完全受到抑制。在2.0 g l(-1)的可耐受初始浓度下，培养4天后，2.0 g l(-1)的SDS中近80%被降解，同时细胞生长增加。计算得出该细菌的烷基硫酸酯酶的K(m(app))和V(max(app))值分别为0.1 mM SDS和1.07 micromol min(-1) mg(-1)蛋白质。
