Syed M A, Sim H K, Khalid A, Shukor M Y
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):89-92.
A stab-culture method was adapted to screen for azo dyes-decolorizing bacteria from soil and water samples. Decolorized azo dye in the lower portion of the solid media indicates the presence of anaerobic azo dyes-decolorizing bacteria, while aerobic decolorizing bacteria decolorizes the surface portion of the solid media. Of twenty soil samples tested, one soil sample shows positive results for the decolourisation of two azo dyes; Biebrich scarlet (BS) and Direct blue 71 (DB) under anaerobic conditions. A gram negative and oxidase negative bacterial isolate was found to be the principal azo dyes degrader The isolate was identified by using the Biolog identification system as Serratia marcescens.
采用穿刺培养法从土壤和水样中筛选偶氮染料脱色菌。固体培养基下部脱色的偶氮染料表明存在厌氧偶氮染料脱色菌，而好氧脱色菌则使固体培养基表面部分脱色。在所测试的20个土壤样品中，有一个土壤样品在厌氧条件下对两种偶氮染料即比布列希猩红（BS）和直接蓝71（DB）的脱色呈阳性结果。发现一株革兰氏阴性且氧化酶阴性的细菌分离株是主要的偶氮染料降解菌。使用Biolog鉴定系统将该分离株鉴定为粘质沙雷氏菌。
