Petric A, Bhat B, Leonard N J, Gumport R I
Department of Chemistry and Chemical Technology, E. Kardelj University, Ljubljana, Yugoslavia.
Nucleic Acids Res. 1991 Feb 11;19(3):585-90. doi: 10.1093/nar/19.3.585.
Compounds that are covalent analogues of nucleic acid base pairs of normal, long, and short C1' to C1' dimensions [B. Devadas and N.J. Leonard (1990) J. Am. Chem. Soc., 112, 3125-3135.] have been added to the oligodeoxyribonucleotide d(A)6 with bacteriophage T4 RNA ligase as a prelude to placing them at defined loci within nucleic acid duplexes. Analogue cross sections that represent a normal Watson-Crick base pair as well as a pyrimidine-pyrimidine and a purine-purine apposition were ligated in modest yields (approximately 20%) to the oligonucleotide. Ligation conditions were optimized for each analogue, and the cross section was joined to only a single oligonucleotide in each case. The structures of the ligated products were proved by HPLC, enzymatic degradation, and spectroscopic analyses.
正常、长和短C1'至C1'尺寸的核酸碱基对的共价类似物[B. Devadas和N.J. Leonard（1990年）《美国化学会志》，112，3125 - 3135]已与噬菌体T4 RNA连接酶一起添加到寡脱氧核糖核苷酸d(A)6中，作为将它们置于核酸双链体内特定位点的前奏。代表正常沃森 - 克里克碱基对以及嘧啶 - 嘧啶和嘌呤 - 嘌呤并列的类似物横截面以适度的产率（约20%）连接到寡核苷酸上。针对每种类似物优化了连接条件，并且在每种情况下横截面仅与单个寡核苷酸连接。通过高效液相色谱、酶促降解和光谱分析证明了连接产物的结构。
