Stepanova Hana, Pavlova Barbora, Stromerova Nikola, Matiasovic Jan, Kaevska Marija, Pavlik Ivo, Faldyna Martin
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Vet Immunol Immunopathol. 2011 Jul 15;142(1-2):107-12. doi: 10.1016/j.vetimm.2011.04.005. Epub 2011 Apr 14.
The zoonotic characteristic of Mycobacterium avium subsp. avium (MAA) represents a veterinary and economic problem in infected pigs. In this study, we analysed cell-mediated immunity six months after experimental infection by measuring interferon-γ (IFN-γ) production and by performing lymphocyte transformation tests after in vitro re-stimulation with the MAA-derived antigen. At the same time, IFN-γ-producing cells were characterised by flow cytometry. In MAA-infected animals, the production of IFN-γ increased in response to the MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by the proliferation assay. In contrast, IFN-γ production and proliferation was undetectable after stimulation with the MAA antigen in uninfected control animals. These results indicate that both methods can be used for the identification of individual MAA-infected pigs. Using flow cytometry, we found that double-positive CD4(+)CD8(+) lymphocytes were the major T lymphocyte subset producing IFN-γ after in vitro re-stimulation.
鸟分枝杆菌鸟亚种（MAA）的人畜共患病特征在感染猪中构成了一个兽医和经济问题。在本研究中，我们通过测量干扰素-γ（IFN-γ）的产生以及在用MAA衍生抗原进行体外再刺激后进行淋巴细胞转化试验，分析了实验感染六个月后的细胞介导免疫。同时，通过流式细胞术对产生IFN-γ的细胞进行了表征。在感染MAA的动物中，血液、脾脏和肠系膜淋巴结中IFN-γ的产生因MAA抗原而增加。同样，增殖试验检测到了阳性的抗原驱动反应。相比之下，在未感染的对照动物中，用MAA抗原刺激后未检测到IFN-γ的产生和增殖。这些结果表明这两种方法均可用于识别个体感染MAA的猪。通过流式细胞术，我们发现双阳性CD4(+)CD8(+)淋巴细胞是体外再刺激后产生IFN-γ的主要T淋巴细胞亚群。
