Barasona Jose A, Barroso-Arévalo Sandra, Rivera Belén, Gortázar Christian, Sánchez-Vizcaíno Jose M
VISAVET Health Surveillance Centre and Animal Health Department, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain.
SaBio. Instituto de Investigación en Recursos Cinegéticos (IREC; CSIC-UCLM), Ronda de Toledo s/n,Ciudad Real 13003, Spain.
Pathogens. 2020 Mar 25;9(4):242. doi: 10.3390/pathogens9040242.
The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC.
牛分枝杆菌及结核分枝杆菌复合群（MTC）其他成员的存在是欧亚野猪（Sus scrofa）等野生动物种群的主要关注点。检测针对MTC抗体的检测方法对于结核病（TB）监测和控制很有价值，在猪科动物中尤其有用。开发准确、高效且非侵入性的检测MTC暴露的新工具对改善疾病监测将非常有益。本研究旨在确定能否通过一种新的酶联免疫吸附测定（ELISA）试验（检测IgG）从自然感染结核病的野猪的口腔液（OF）样本中检测到针对MTC的抗体。为此，从西班牙两个结核病状态区域的148头活体野猪中采集个体、配对的血清和OF样本，并定量用于验证新的ELISA试验。在OF样本中广泛检测到针对MTC的抗体，其与经过验证的血清学检测呈显著正相关（r = 0.83）。OF ELISA的敏感性和特异性分别为67.3%和100%。这项工作的结果表明，OF样本有潜力用于MTC诊断，作为猪科动物种群结核病监测和控制的进一步措施。基于我们的结果，有必要进行进一步研究，可以直接在野外条件下使用非侵入性新工具来检测MTC暴露情况。
