Fawzy A, Fayed A, Youssef H, El-Sayed A, Zschöck M
Ph.D. Student in Animal Infectious Diseases, Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; ; Hessian State Laboratory (LHL), Giessen, Germany.
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Iran J Vet Res. 2016 Spring;17(2):130-133.
subsp. (MAP) is the causative agent of Johne's disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different countries. Until now there is no published information about MIRU-VNTR genotyping of MAP isolates in Egypt. To address that point, 100 faecal samples were collected and cultivated from 3 different suspected dairy farms. Fourteen isolates belonging to one farm were identified as MAP and subjected to genotyping using 8 different MIRU-VNTR loci PCRs. Two different genotypes were recognized based on size polymorphism observed in one locus (VNTR-7) that was confirmed by sequencing. Our work provides a preliminary basis of constructing a MIRU-VNTR genotyping database of MAP in Egypt.
副结核分枝杆菌亚种（MAP）是牛副结核病的病原体，牛副结核病是一种在全球范围内对反刍动物具有重要经济影响的疾病。它于2005年首次在埃及分离出来。从那时起，在埃及的不同省份都检测到了这种病原体。为了追踪感染源，不同国家采用了分枝杆菌散布重复单位-可变数目串联重复序列（MIRU-VNTR）等高鉴别力的简单方法进行基因分型。到目前为止，还没有关于埃及MAP分离株MIRU-VNTR基因分型的公开信息。为了解决这一问题，从3个不同的疑似奶牛场收集并培养了100份粪便样本。属于一个农场的14株分离株被鉴定为MAP，并使用8个不同的MIRU-VNTR位点PCR进行基因分型。基于在一个位点（VNTR-7）观察到的大小多态性识别出两种不同的基因型，测序证实了这一点。我们的工作为构建埃及MAP的MIRU-VNTR基因分型数据库提供了初步基础。
