Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Argentina.
Benef Microbes. 2011 Mar;2(1):29-34. doi: 10.3920/BM2010.0024.
Lactobacillus johnsonii CRL1647, isolated from the intestinal tract of a honeybee and selected due to its high lactic acid production, was assayed as a monoculture on bee colony performance. It was delivered to the bees on a one litre of 125 g/l sugar-cane syrup with a final concentration of 105 cfu/ml lactobacilli. The bees accepted the new nourishment, which was consumed within 24-48 h and was administered in two independent trials (every 14-15 days for 3 consecutive months in one case, and once a month for 13 consecutive months in the other). From late spring - early summer (2008) the photo-records and statistical analyses revealed significant differences in the open and the operculated brood areas in the treated group compared with the control. This stimulation was observed after the first administration of the lactobacilli and maintained throughout. Also, a higher number of bees were measured in the treated group (54%) and the control (18%) with respect to the initial bees' number. Furthermore, honey storage was higher, 40% and 19%, for the treated and control groups, respectively. From December 2008 to December 2009, a similar situation was observed even though, in this trial, the lactobacilli cells were administered once a month. The in vivo results of this study are promising and indicate that a L. johnsonii CRL1647 supplement to beehives favours mainly open and operculated brood areas, demonstrating a stronger stimulation of egg-laying and will become a natural product which will assist the beekeeper both in colony management and the creation of late nuclei and/or bee packages due to its beneficial effects in the beehive colony.
从蜜蜂肠道中分离出的高产乳酸的约翰逊乳杆菌 CRL1647，作为单一培养物对蜂群性能进行了检测。将其添加到 1 升 125g/L 的甘蔗糖浆中，最终乳酸杆菌的浓度为 105cfu/ml。蜜蜂接受了新的营养物质，在 24-48 小时内被消耗完，并且在两个独立的试验中进行了管理（在一个试验中，连续 3 个月每隔 14-15 天给药一次，在另一个试验中，连续 13 个月每月给药一次）。从春末到初夏（2008 年），照片记录和统计分析显示，实验组的开放式和封盖式幼虫区与对照组相比有显著差异。这种刺激在第一次给药后观察到，并持续存在。此外，实验组（54%）和对照组（18%）的蜜蜂数量均高于初始蜜蜂数量。此外，实验组的蜂蜜储存量更高，为 40%和 19%，分别为实验组和对照组。从 2008 年 12 月到 2009 年 12 月，即使在这个试验中，每月只给一次乳酸杆菌细胞，也观察到了类似的情况。这项研究的体内结果很有希望，表明向蜂群中补充约翰逊乳杆菌 CRL1647 主要有利于开放式和封盖式幼虫区，这表明对产卵的刺激更强，并且由于其对蜂群的有益作用，将成为一种天然产品，这将有助于养蜂人管理蜂群，并创造后期的核心群和/或蜜蜂群。
