Center for Animal Disease Research and Diagnosis, Pathology Laboratory, Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, India.
Vaccine. 2010 Mar 16;28(13):2522-31. doi: 10.1016/j.vaccine.2010.01.039. Epub 2010 Jan 29.
Bluetongue is a serious hemorrhagic disease of sheep, cattle and other ruminants causing economic losses worldwide. Recent invasion of multiple bluetongue virus serotypes (BTV) in various countries warrants immediate development of efficacious vaccine that targets more than one serotype. In the present study, the cross-protective efficacy of binary ethylenimine (BEI)-inactivated BTV-1 vaccine was evaluated in Indian native sheep against virulent heterologous BTV-23 serotype challenge. BTV-1 vaccination induced significant cell-mediated immunity (CMI) as determined by lymphoproliferative responses, and increased CD8 T cell, IL-2 and IFN-gamma responses. Both naïve and immunized sheep also showed increased CD4 T cell, IL-12 and IFN-alpha responses. Collectively, these data suggested that inactivated BTV-1 vaccine induced appreciable CMI and greatly reduced the severity of heterologous BTV-23 infection.
蓝舌病是一种严重的绵羊、牛和其他反刍动物的出血性疾病，在世界范围内造成了经济损失。最近，多种蓝舌病病毒血清型（BTV）在不同国家的入侵，需要立即开发针对多种血清型的有效疫苗。在本研究中，用双乙烯亚胺（BEI）灭活的 BTV-1 疫苗对印度本土绵羊进行了评估，以对抗强毒异源 BTV-23 血清型的挑战。BTV-1 疫苗接种诱导了显著的细胞介导免疫（CMI），如淋巴细胞增殖反应所确定的，并且增加了 CD8 T 细胞、IL-2 和 IFN-γ反应。未免疫和免疫的绵羊也表现出增加的 CD4 T 细胞、IL-12 和 IFN-α反应。总的来说，这些数据表明，灭活的 BTV-1 疫苗诱导了可观的 CMI，并大大减轻了异源 BTV-23 感染的严重程度。
