口服脂肪组织来源的混合抗原对动脉粥样硬化和肥胖指数的影响。

Effect of oral immunization with pooled antigens derived from adipose tissue on atherosclerosis and obesity indices.

机构信息

Immunitor USA Inc., hq 9308 48th avenue, College Park, MD 20740, USA.

出版信息

Vaccine. 2010 Mar 24;28(15):2763-8. doi: 10.1016/j.vaccine.2010.01.032. Epub 2010 Jan 29.

DOI:10.1016/j.vaccine.2010.01.032

PMID:20117273

Abstract

Modulation of the inflammatory response through vaccination has shown promise in animal models of atherogenesis and obesity-main risk factors for cardiovascular diseases. Tableted preparation, V-6, containing pooled antigens derived from pig adipose tissue was administered per os daily to 13 volunteers for 3 months. Total cholesterol and LDL levels have not changed from the baseline value; 193.4 vs 191.8 (p=0.75) and 113.8 vs 117.2 (p=0.18), respectively. Triglyceride levels declined by 26.1% from 163 to 120.5mg/dL (p=0.29). HDL increased by 25.9% from 38.6 to 48.5mg/dL (p=0.000002) in 12 out 13 patients. Despite negligible effect of V6 on weight and body mass index, i.e., 67.87 vs 66.58 kg (-1.9%; p=0.46) and 26.25 vs 25.75 kg/m(2) (-1.9%; p=0.35), statistically significant reduction in waist (-7.6%; p=0.002), mid-arm (-3.3%; p=0.049), and thigh (-7.6%; p=0.0003) circumferences were observed. Blood pressure systolic and diastolic values were not affected, i.e., 115.6 vs 121 (p=0.2) and 77.1 vs 83 (p=0.55). No changes were observed in liver ALT and AST enzymes. Creatinine decreased; 0.877 vs 0.842 mg/dL (p=0.02), while BUN has shown slight increase; 14.8 vs 16.1mg/dL (p=0.03) but within normal range. Fasting blood sugar levels were also within normal range, i.e., 94.8 vs 98.8 (p=0.04). Complete blood cell analysis has not revealed any change except slight decrease in hemoglobin 13.25-13.19 g/dL (p=0.0004), but its amount per red blood cell (MCH) increased from 25.62 up to 26.42 picograms/cell (p=0.000003). The average red blood cell size (MCV) increased from 78.5 to 80.0 fl (p=0.00009) but hemoglobin concentration relative to size of the cell (MCHC) has not changed (p=0.2). Hematocrit and red blood cells count decreased slightly, but within normal range 40.7-40.2% (p=0.02) and 5.22-5.05 x 10(6)cells/mm(3) (p=0.002). The number of platelets moved upward, 2.471 vs 2.921 x 10(5)per mm(3) (p=0.009). The white blood cells count and percent of leukocytes and neutrophils were not affected. Pro-inflammatory eosinophils declined from 4.0% down to 2.4% (p=0.29). These results show that V6 is safe and holds a promise as an anti-atherogenic and overweight/obesity immune intervention.

摘要

口服含有猪脂肪组织来源的混合抗原的 V-6 片剂制剂，每日一次，共 13 名志愿者连续服用 3 个月。总胆固醇和 LDL 水平从基线值没有变化；分别为 193.4 与 191.8（p=0.75）和 113.8 与 117.2（p=0.18）。甘油三酯水平从 163 降至 120.5mg/dL，下降了 26.1%（p=0.29）。13 名患者中有 12 名的高密度脂蛋白增加了 25.9%，从 38.6 升至 48.5mg/dL（p=0.000002）。尽管 V6 对体重和体重指数几乎没有影响，即 67.87 与 66.58kg（-1.9%；p=0.46）和 26.25 与 25.75kg/m2（-1.9%；p=0.35），但腰围（-7.6%；p=0.002）、上臂中部（-3.3%；p=0.049）和大腿（-7.6%；p=0.0003）的周长却明显减小。血压的收缩压和舒张压没有变化，分别为 115.6 与 121（p=0.2）和 77.1 与 83（p=0.55）。肝丙氨酸转氨酶和天冬氨酸转氨酶活性没有变化。肌酸酐降低；0.877 与 0.842mg/dL（p=0.02），而血尿素氮略有增加；14.8 与 16.1mg/dL（p=0.03），但仍在正常范围内。空腹血糖水平也在正常范围内，即 94.8 与 98.8（p=0.04）。全血细胞分析除血红蛋白略有下降外（13.25-13.19g/dL；p=0.0004），未发生其他变化，但每个红细胞的平均血红蛋白含量（MCH）从 25.62 增加到 26.42 皮克/细胞（p=0.000003）。平均红细胞体积（MCV）从 78.5 增加到 80.0fl（p=0.00009），但血红蛋白浓度与细胞大小的比值（MCHC）没有变化（p=0.2）。红细胞压积和红细胞计数略有下降，但仍在正常范围内（分别为 40.7-40.2%；p=0.02 和 5.22-5.05 x 106cells/mm3；p=0.002）。血小板数量上升，为 2.471 与 2.921 x 105/mm3（p=0.009）。白细胞计数、白细胞百分比和中性粒细胞百分比不受影响。促炎嗜酸性粒细胞从 4.0%下降到 2.4%（p=0.29）。这些结果表明，V6 是安全的，具有作为抗动脉粥样硬化和超重/肥胖免疫干预的潜力。