Mo Yu-jing, Huang Wen-hui, Chen Dong-li, Chen Fu-rong
Guangdong Cardovascular Institute, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jan;30(1):84-7.
To study the relationship between angiotensin-converting enzyme 2 (ACE2) gene G9570A polymorphisms and the clinical outcome of stroke patients with essential hypertension (EH) in South China Han population.
The ACE2 gene polymorphisms were detected in 141 stroke patients with EH and 156 patients with EH using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genetic marker was tested for its association with the baseline measurements and clinical outcomes of the patients over a median follow-up period of 22 months. As the ACE2 gene is X-linked, analyses were performed for male and female patients separately.
The A allele frequency in the stroke patients was significantly different from that in the EH patients, and the AA allele frequency in the female patients was significantly different between the two groups (P<0.01). Kaplan-Meier model analysis showed that ACE2 gene polymorphism was not associated with the the patients' prognosis (P>0.05). Multivariate Cox's proportional hazard regression model identified age (RR=1.057, 95%CI: 1.020, 1.095), blood glucose (RR=1.575, 95%CI: 1.178, 2.104), hypertriglyceridemia (RR=1.947, 95%CI: 1.503, 2.780), blood creatinine (RR=1.034, 95%CI: 1.001, 1.068), and blood uric acid (RR=1.056, 95%CI: 1.002, 1.097) as the risk factors associated with the mortality.
Stroke occurs more likely in hypertensive patients carrying the A/AA allele than those carrying other alleles. The ACE2 gene G9570A polymorphisms may be associated with the occurrence of stroke in EH patients in South China, but may not have a strong correlation to the prognosis.
研究血管紧张素转换酶2（ACE2）基因G9570A多态性与中国南方汉族原发性高血压（EH）中风患者临床结局的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测141例EH中风患者和156例EH患者的ACE2基因多态性。在中位随访期22个月内，检测该基因标记与患者基线测量值及临床结局的相关性。由于ACE2基因是X连锁的，因此分别对男性和女性患者进行分析。
中风患者的A等位基因频率与EH患者显著不同，两组女性患者的AA等位基因频率也有显著差异（P<0.01）。Kaplan-Meier模型分析显示，ACE2基因多态性与患者预后无关（P>0.05）。多变量Cox比例风险回归模型确定年龄（RR=1.057，95%CI：1.020，1.095）、血糖（RR=1.575，95%CI：1.178，2.104）、高甘油三酯血症（RR=1.947，95%CI：1.503，2.780）、血肌酐（RR=1.034，95%CI：1.001，1.068）和血尿酸（RR=1.056，95%CI：1.002，1.097）为与死亡率相关的危险因素。
携带A/AA等位基因的高血压患者比携带其他等位基因的患者更容易发生中风。ACE2基因G9570A多态性可能与中国南方EH患者中风的发生有关，但与预后可能没有很强的相关性。
