Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2979-2983. doi: 10.1099/ijs.0.021527-0. Epub 2010 Jan 29.
A bacterial strain designated TNR-18(T) was isolated from stream water in southern Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TNR-18(T) were Gram-stain-negative, strictly aerobic, non-motile and vibrioid, and the strain formed orange colonies. Growth occurred at 10-30°C, with 0-0.2 % NaCl and at pH6.0-10.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-18(T) belonged to the genus Arcicella; its closest neighbours were Arcicella rosea TW5(T) and Arcicella aquatica NO-502(T), with respective sequence similarities of 97.9 and 97.8 %. Predominant cellular fatty acids (>10 %) were summed feature 3 (C(16 : 1)ω7c/C(16 : 1)ω6c; 37.7 %), C(16 : 1)ω5c (13.0 %) and iso-C(15 : 0) (11.1 %). The major respiratory quinone was MK-7. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized polar lipids. The DNA G+C content was 39.9 mol%. On the basis of the phylogenetic and phenotypic data, strain TNR-18(T) should be classified as representing a novel species, for which the name Arcicella aurantiaca sp. nov. is proposed. The type strain is TNR-18(T) (=LMG 25207(T) =BCRC 17969(T)).
一株名为 TNR-18(T) 的细菌菌株从台湾南部的溪流水中分离出来，并通过多相分类学方法进行了特征描述。菌株 TNR-18(T) 的细胞呈革兰氏阴性，严格需氧，不运动，呈弧菌状，形成橙色菌落。该菌株在 10-30°C、0-0.2%NaCl 和 pH6.0-10.0 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 TNR-18(T) 属于 Arcicella 属；与 Arcicella rosea TW5(T) 和 Arcicella aquatica NO-502(T) 的亲缘关系最近，序列相似度分别为 97.9%和 97.8%。主要细胞脂肪酸（>10%）为(summed feature 3，C(16:1)ω7c/C(16:1)ω6c；37.7%)、C(16:1)ω5c（13.0%）和 iso-C(15:0)（11.1%）。主要呼吸醌为 MK-7。极性脂谱含有磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和几种未鉴定的极性脂。DNA G+C 含量为 39.9 mol%。基于系统发育和表型数据，菌株 TNR-18(T) 应归类为代表一个新种，因此提议将其命名为 Arcicella aurantiaca sp. nov.。模式菌株为 TNR-18(T)（=LMG 25207(T)=BCRC 17969(T)）。
