热休克蛋白 60 与 16S rRNA 基因序列关系在类杆菌属的种间发现，猪型拟杆菌和产真杆菌是多形拟杆菌的异名。
hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes.
机构信息
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Benno Laboratory, Center for Intellectual Property Strategies, RIKEN, Wako, Saitama 351-0198, Japan.
出版信息
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2984-2990. doi: 10.1099/ijs.0.021154-0. Epub 2010 Jan 29.
hsp60 gene sequences were determined for members of the genus Bacteroides and sequence similarities were compared with those obtained for the 16S rRNA gene. Among the 29 Bacteroides type strains, the mean sequence similarity of the hsp60 gene (84.5 %) was significantly less than that of the 16S rRNA gene (90.7 %), indicating a high discriminatory power of the hsp60 gene. Species of the genus Bacteroides were differentiated well by hsp60 gene sequence analysis, except for Bacteroides pyogenes JCM 6294(T), Bacteroides suis JCM 6292(T) and Bacteroides tectus JCM 10003(T). The hsp60 gene sequence analysis and the levels of DNA-DNA relatedness observed demonstrated that these three type strains are a single species. Consequently, B. suis and B. tectus are heterotypic synonyms of B. pyogenes. This study suggests that the hsp60 gene is an alternative phylogenetic marker for the classification of species of the genus Bacteroides.
hsp60 基因序列被确定为拟杆菌属的成员，并与 16S rRNA 基因的序列相似性进行了比较。在 29 株拟杆菌型菌株中，hsp60 基因的平均序列相似性（84.5%）明显低于 16S rRNA 基因（90.7%），表明 hsp60 基因具有很高的鉴别能力。除了化脓拟杆菌 JCM 6294(T)、猪拟杆菌 JCM 6292(T)和 Tectus 拟杆菌 JCM 10003(T)外，拟杆菌属的物种通过 hsp60 基因序列分析得到很好的区分。hsp60 基因序列分析和观察到的 DNA-DNA 相关水平表明，这三个模式株是一个单一的物种。因此，猪拟杆菌和 Tectus 拟杆菌是化脓拟杆菌的异名。本研究表明，hsp60 基因是拟杆菌属物种分类的替代系统发育标记。
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